P4020

Sigma-Aldrich

Protamine sulfate salt from salmon

Grade X, amorphous powder

Sinónimos:
Salmine from salmon, Salmine
Número de CAS:
MDL number:
NACRES:
NA.77

Quality Level

type

Grade X

form

amorphous powder

impurities

histone, free (Millon test)

color

white to off-white

solubility

6 M HCl: 25 mg/mL

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General description

Protamine are proteins rich in cysteine and arginine. It possesses many phosphorylation sites. It is found to be localized to the sperm head.

Application

Protamine sulfate salt from salmon has been used:
  • in the pretreament of cells to study its effect on respiratory syncytial virus infection
  • in lentivirus production
  • to derivatize it into a diazeniumdiolate NO donor

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Packaging

1, 5, 10, 25, 100 g in poly bottle

Biochem/physiol Actions

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

The design and evaluation of heparin-binding foldamers.
Sungwook Choi et al.
Angewandte Chemie (International ed. in English), 44(41), 6685-6689 (2005-08-12)
Microarrays of lentiviruses for gene function screens in immortalized and primary cells
Bailey SN, et al.
Nature Methods, 3(2), 117-117 (2006)
Wenjie Ouyang et al.
Human gene therapy, 32(9-10), 481-494 (2020-12-02)
β-Thalassemia is one of the most prevalent genetic diseases worldwide. The current treatment for β-thalassemia is allogeneic hematopoietic stem cell transplantation, which is limited due to lack of matched donors. Gene therapy has been developed as an alternative therapeutic option...
Steve N Bailey et al.
Nature methods, 3(2), 117-122 (2006-01-25)
Here we describe lentivirus-infected cell microarrays for the high-throughput screening of gene function in mammalian cells. To create these arrays, we cultured mammalian cells on glass slides 'printed' with lentiviruses pseudotyped as vesicular stomatitis virus glycoprotein, which encode short hairpin...
Iduronic acid-containing glycosaminoglycans on target cells are required for efficient respiratory syncytial virus infection
Hallak LK, et al.
Virology, 271(2), 264-275 (2000)

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