Poly-L-lysine solution

0.01%, sterile-filtered, BioReagent, suitable for cell culture

PLL Solution
Número de CAS:
MDL number:

Quality Level



product line





pkg of 50 mL




cell culture | mammalian: suitable


endotoxin, tested

shipped in


storage temp.




InChI key


¿Está buscando productos similares? Visit Guía de comparación de productos

Categorías relacionadas


  • Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.
  • Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.
  • Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.


Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.


12 - Non Combustible Liquids

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis

Certificado de origen

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of Product P4707, Poly-L-Lysine solution?

    This product is supplied as a 0.01% w/v solution.

  4. What molecular weight Poly-L-Lysine product should I choose?

    The lower molecular weight poly-L-lysine (30-70 kDa) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kDa) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kDa.

  5. Should I use Poly-L-Lysine or Poly-D-Lysine?

    Certain cells secrete proteases, which can digest poly-L-lysine. For those cell types, poly-D-lysine should be used.

  6. What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat slides?

    For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. After a 5 minute incubation, the excess solution is removed and the slides are dried at room temperature or in an oven at gentle heat.

  7. What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat tissue culture plasticware?

    A solution of 0.1 mg/mL is prepared; 0.5 mL of that solution is aseptically transferred to a non-tissue culture treated 25 square cm flask. The flask is gently rocked to evenly coat the surface. After five minutes, the excess solution is removed and the surface is thoroughly rinsed with sterile water and allowed to dry for several hours.

  8. How can I sterilize slides coated with Product P4707, Poly-L-Lysine solution?

    Poly-L-Lysine coated slides can be exposed to UV light overnight for sterilization.

  9. How should I dilute Product P4707, Poly-L-Lysine solution?

    For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution.  However, if your protocol requires a more dilute solution, you can add sterile water or sterile buffer like Phosphate Buffered Saline.

  10. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

All polymer chip for amperometric studies of transmitter release from large groups of neuronal cells.
Larsen ST and Taboryski R
Analyst, 137, 5057-5057 (2012)
Promiscuous dimerization of the growth hormone secretagogue receptor (GHS-R1a) attenuates ghrelin-mediated signaling.
Schellekens H et al.
The Journal of Biological Chemistry, 288, 181-181 (2013)
Microparticles mediate MRP1 intercellular transfer and the re-templating of intrinsic resistance pathways.
Lu JF et al.
Pharmacological Research, 76, 77-77 (2013)
Alexandre Stipanovich et al.
Nature, 453(7197), 879-884 (2008-05-23)
Dopamine orchestrates motor behaviour and reward-driven learning. Perturbations of dopamine signalling have been implicated in several neurological and psychiatric disorders, and in drug addiction. The actions of dopamine are mediated in part by the regulation of gene expression in the...
Vivian S W Li et al.
Cell, 149(6), 1245-1256 (2012-06-12)
Degradation of cytosolic β-catenin by the APC/Axin1 destruction complex represents the key regulated step of the Wnt pathway. It is incompletely understood how the Axin1 complex exerts its Wnt-regulated function. Here, we examine the mechanism of Wnt signaling under endogenous...
Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.
Más información
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Más información
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Más información
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Más información
Polyamino acids facilitate the attachment of cells and proteins to solid surfaces in biological applications. In cell cultures normal attachment, growth, and development of many cell types are dependent on attachment factors and extracellular matrix components.
Más información

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico