pkg of 50 mL
cell culture | mammalian: suitable
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12 - Non Combustible Liquids
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
This product is supplied as a 0.01% w/v solution.
The lower molecular weight poly-L-lysine (30-70 kDa) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kDa) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kDa.
Certain cells secrete proteases, which can digest poly-L-lysine. For those cell types, poly-D-lysine should be used.
For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. After a 5 minute incubation, the excess solution is removed and the slides are dried at room temperature or in an oven at gentle heat.
A solution of 0.1 mg/mL is prepared; 0.5 mL of that solution is aseptically transferred to a non-tissue culture treated 25 square cm flask. The flask is gently rocked to evenly coat the surface. After five minutes, the excess solution is removed and the surface is thoroughly rinsed with sterile water and allowed to dry for several hours.
Poly-L-Lysine coated slides can be exposed to UV light overnight for sterilization.
For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. However, if your protocol requires a more dilute solution, you can add sterile water or sterile buffer like Phosphate Buffered Saline.
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