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P5380

Sigma-Aldrich

Protease from Bacillus licheniformis

Type VIII, lyophilized powder, 7-15 units/mg solid

Synonym(s):
Proteinase from Bacillus licheniformis, Subtilisin A
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
NACRES:
NA.54

origen biológico

Bacillus licheniformis

Nivel de calidad

200

tpo

Type VIII

formulario

lyophilized powder

specific activity

7-15 units/mg solid

mol peso

27 kDa

purificado por

crystallization

solubilidad

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear
H2O: soluble, colorless

actividad extraña

DNase ≤5.0 Kunitz units/mg solid
RNase ≤0.05 Kunitz units/mg solid

temp. de almacenamiento

−20°C

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Descripción general

The enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60 °C.

Especificidad

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Aplicación

The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination.[1] It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies.[2]
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.

Envase

25 mg in poly bottle
100, 250 mg in glass bottle
1 g in glass bottle

Acciones bioquímicas o fisiológicas

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease, Product P5380, is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is active in some organic solvents such as dry octane.

Propiedades físicas

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Definición de unidad

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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