Merck
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P7405

Sigma-Aldrich

Poly-D-lysine hydrobromide

mol wt >300,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Synonym(s):
PDL
Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.75

Nivel de calidad

esterilidad

γ-irradiated

Línea del producto

BioReagent
BioXtra

formulario

lyophilized powder

mol peso

>300,000

envase

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

solubilidad

H2O: soluble 50 mg/mL, clear, colorless

enviado en

ambient

temp. de almacenamiento

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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This Item
P0296P6407P7886
sterility

γ-irradiated

sterility

-

sterility

γ-irradiated

sterility

-

form

lyophilized powder

form

powder or solid

form

lyophilized powder

form

solid

mol wt

>300,000

mol wt

1,000-5,000

mol wt

70,000-150,000

mol wt

30,000-70,000

packaging

pkg of 5 mg

packaging

-

packaging

pkg of 10 X 5 mg, pkg of 5 mg

packaging

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 50 mg/mL, clear, colorless

solubility

-

solubility

H2O: soluble 50 mg/mL, clear, colorless

solubility

-

Aplicación

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Acciones bioquímicas o fisiológicas

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Componentes

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Precaución

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Nota de análisis

This product has a molecular weight >300,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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