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Merck

P7493

Sigma-Aldrich

Anti-PCAF antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-p300/CBP associated factor

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~88 kDa

species reactivity

canine, human

technique(s)

immunoprecipitation (IP): 5-10 μg using RIPA lysate with ~4x107 human acute T cell leukemia Jurkat cells
microarray: suitable
western blot (chemiluminescent): 1:500 using whole extracts of dog MDCK kidney cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KAT2B(8850)

Categorías relacionadas

General description

PCAF (p300/CBP associated factor, P/CAF), a type A histone acetyltransferases (HAT), has been identified in several species. Human PCAF is a ubiquitous protein member of the GCN5-related N-acetyltransferase (GNAT) superfamily.

Specificity

Anti-PCAF recognizes human and dog PCAF. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.

Immunogen

This sequence is identical to mouse PCAF and varies by 2 amino acids residues in chicken.
synthetic peptide corresponding to the C-terminal (amino acids 817-832) of human PCAF with N-terminal added cysteine, conjugated to KLH.

Application

Anti-PCAF antibody is also suitable for use microarray, immunoblot, chemiluminescent western blot (1:500 using whole extracts of dog MDCK kidney cells), and immunoprecipitation (5-10 μg using RIPA lysate with approx. 4x10,000,000 human acute T cell leukemia Jurkat cells).
Anti-PCAF antibody produced in rabbit has been used in western blot and immunoprecipitation.

Biochem/physiol Actions

PACF (p300/CBP association factor) functions as a transcriptional coactivator in association with p300 and CBP (CREB-binding protein). PCAF share high degree of sequence similarity with human and mouse GCN5 HAT (Histone acetyltransferases). PACF mediates the acetylation of core histone. It is also known for the acetylation of non-histone proteins such as p53, MyoD, TFIIE-b, TFIIE, GATA-1, EKLF, HMGI (Y), and several other cellular and viral proteins. PCAF possesses chromatin modification activity and modulation of transcription factors for the activation of transcription. It also promotes cell growth and development as myogenesis and also contribute to receptor-mediated cell signaling or activation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Differential Effects of Histone Acetyltransferase GCN5 or PCAF Knockdown on Urothelial Carcinoma Cells
Koutsogiannouli EA, et al.
International Journal of Molecular Sciences, 18, 7-7 (2017)
Target gene context influences the transcriptional requirement for the KAT3 family of CBP and p300 histone acetyltransferases
Bedford DC, et al.
Epigenetics, 5(1), 9-15 (2010)
Transcriptional/epigenetic regulator CBP/p300 in tumorigenesis: structural and functional versatility in target recognition
Wang F, et al.
Cellular and Molecular Life Sciences, 70(21), 3989-4008 (2013)
Nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase is regulated by acetylation
Ventura M, et al.
The International Journal of Biochemistry & Cell Biology, 42(10), 1672-1680 (2010)
Is histone acetylation the most important physiological function for CBP and p300?
Bedford DC and Brindle PK
Aging (Albany. NY.), 4(4), 247-247 (2012)

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