Merck
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P7582

Sigma-Aldrich

Amino-terminal FLAG-BAP Fusion Protein

Número MDL:
NACRES:
NA.32

formulario

liquid

Nivel de calidad

200

mol peso

~49 kDa

enviado en

dry ice

temp. de almacenamiento

−20°C

Related Categories

Descripción general

Amino-terminal FLAG-BAP Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.[1]
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Aplicación

Amino-terminal FLAG-BAP Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification[2] and in western blotting based quantification of Flag-Wnt8[3] and Drosophila melanogaster transcription factor interactome.[4]
Browse additional application references in our FLAG® Literature portal.

Otras notas

Control protein

Forma física

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Información legal

FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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