Merck

P7624

Sigma-Aldrich

Anti-Profilin 1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Sinónimos:
Anti-PFN1
Número MDL:
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

antibody product type

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen ~15 kDa

species reactivity

mouse, human, rat

concentración

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using mouse NIH3T3 cell lysates
indirect immunofluorescence: 10-20 μg/mL using rat NRK cells
western blot (chemiluminescent): 1-2 μg/mL using whole extract of human HeLa cells

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

Gene Information

human ... PFN1(5216)
mouse ... Pfn1(18643)
rat ... Pfn1(64303)

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Este artículo
P7749SAB4200357P0101
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

IgG fraction of antiserum

clone

polyclonal

clone

polyclonal

clone

Profilin 1-3, monoclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen ~15 kDa

mol wt

antigen ~15 kDa

mol wt

antigen ~15 kDa

mol wt

antigen ~14 kDa

Descripción general

Profilin 1 is a ubiquitous actin monomer-binding protein. Profilin 1 is the most ubiquitous and abundant and is highly expressed throughout development and adulthood in most tissues including brain.

Inmunógeno

synthetic peptide corresponding to amino acid residues 128-140 of human profilin 1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Aplicación

Anti-Profilin 1 (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunostaining
  • immunoprecipitation
  • immunofluorescence
  • profilin enzyme-linked immunosorbent assay (ELISA) analysis

Acciones bioquímicas o fisiológicas

Profilin 1 is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Deletion of profilin 1 gene leads to an embryonic lethal phenotype. Profilin 1 is a potent regulator of actin filament dynamics. Although profilin 1 prevents spontaneous actin polymerization by complexing with unpolymerized actin in vivo, actin-profilin complexes can be added to free barbed ends, thereby stimulating actin polymerization. Profilin 1 was suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over-expressed in breast cancer cells. Deletion of profilin 1 is associated with Miller-Dieker syndrome.

Descripción de destino

Profilin 1 is a ubiquitous actin monomer-binding proteininvolved in actin polymerization in response toextracellular signals.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Ruping Wang et al.
Cells, 11(15) (2022-08-13)
Airway smooth muscle cell migration plays an essential role in airway development, repair, and remodeling. Smooth muscle myosin II has been traditionally thought to localize in the cytoplasm solely and regulates cell migration by affecting stress fiber formation and focal
Profilin1 Biology and its Mutation, Actin(g) in Disease
Alkam D, et al.
Cellular and Molecular Life Sciences, 74(6), 967-967 (2017)
Jitendra Kumar Kanaujiya et al.
Proteomics, 13(14), 2100-2112 (2013-04-12)
Nuclear receptor coregulators play an important role in the transcriptional regulation of nuclear receptors. In the present study, we aimed to identify estrogen receptor α (ERα) interacting proteins in Tamoxifen treated MCF7 cells. Using in vitro GST-pull down assay with
Kristin Michaelsen-Preusse et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(12), 3365-3370 (2016-03-10)
Learning and memory, to a large extent, depend on functional changes at synapses. Actin dynamics orchestrate the formation of synapses, as well as their stabilization, and the ability to undergo plastic changes. Hence, profilins are of key interest as they
Dynamic actin structures stabilized by profilin
Finkel T, et al.
Proceedings of the National Academy of Sciences of the USA, 91(4), 1510-1510 (1994)

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