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P7886

Poli-D-lisina hydrobromide

suitable for cell culture, Mol wt 30,000-70,000

Sinónimos:

D-Lysine homopolymer hydrobromide

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Tamaño de envaseSKUDisponibilidadPrecio
10 mg
Comprobar disponibilidad del carrito
MXP 1,187.00
50 mg

Disponible para enviar HOYdesdeToluca

MXP 3,380.00
100 mg

Disponible para enviar HOYdesdeToluca

MXP 5,665.00
500 mg
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MXP 22,597.00
1 g
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MXP 34,294.00

Acerca de este artículo

Fórmula lineal:
D-Lys-(D-Lys)n-D-Lys · xHBr
Número CAS:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.26
MDL number:

MXP 1,187.00


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Nombre del producto

Poli-D-lisina hydrobromide, mol wt 30,000-70,000

form

solid

Quality Level

mol wt

30,000-70,000

technique(s)

cell culture | mammalian: suitable

color

white to off-white

storage temp.

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

Application

Los polímeros de poli-D-lisina pueden utilizarse en la preparación de las superficies para fijación celular. Los polímeros de D-lisina también pueden utilizarse con células que digieren los polímeros poli-L-lisina y causan un captación excesiva de L-lisina.

Este producto está recomendado como sustrato de cultivo celular cuando se utilizan entre 0,5 y 1,0 ml de una disolución 0,1 mg/ml para recubrir 25 cm2. Las versiones del producto de menor peso molecular son menos viscosas, pero las versiones de gran peso molecular proporcionan mas sitios de fijación por molécula.

Biochem/physiol Actions

El hidrobromuro de poli-D-lisina es un factor de unión inespecífica para las células, útil en la promoción de la adhesión celular a los sustratos sólidos al intensificar la interacción electrostática entre iones con carga negativa de la membrana celular y la superficie del cultivo. Después de la absorción a la superficie del cultivo, la poli-D-lisina aumenta el número de sitios de unión a la célula con carga positiva.

Preparation Note

To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Other Notes

La poli-D-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-D-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura β porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.

Disclaimer

Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.

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Este artículo
P0899P6403P0296
form

solid

form

solid

form

powder or solid

form

powder or solid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

color

white to off-white

color

white to off-white

color

white to light yellow

color

white to faint yellow

mol wt

30,000-70,000

mol wt

70,000-150,000

mol wt

4,000-15,000

mol wt

1,000-5,000


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Artículos

Humankind has utilized protein materials throughout its existence, starting with the use of materials such as wool and silk for warmth and protection from the elements and continuing with the use of recombinant DNA techniques to synthesize proteins with unique and useful properties.


Louise Gorham et al.
European journal of pharmacology, 736, 101-106 (2014-04-29)
Somatostatin has a wide biological profile resulting from its actions on the five receptor subtypes (sst1-5). Recently somatostatin was shown to exert analgesic effects via activation of the sst4 receptor. Although the analgesia in pain models is established, the precise
Mandi E Schmidt et al.
BMC biology, 16(1), 58-58 (2018-06-28)
Huntington disease (HD) is a fatal neurodegenerative disorder caused by a CAG expansion in the huntingtin (HTT) gene, leading to selective and progressive neuronal death predominantly in the striatum. Mutant HTT expression causes dysfunctional cortico-striatal (CS) transmission, loss of CS
Michael Brunk et al.
Scientific reports, 8(1), 605-605 (2018-01-14)
The dynamics of early fungal development and its interference with physiological signals and environmental factors is yet poorly understood. Especially computational analysis tools for the evaluation of the process of early spore germination and germ tube formation are still lacking.



Número de artículo de comercio global

SKUGTIN
P7886-500MG04061834398660
P7886-1G04061826757772
P7886-100MG04061834398431
P7886-10MG04061834398653
P7886-50MG04061834400462

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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