P7890

Poli-L-lisina hydrobromide

mol wt 15,000-30,000 by viscosity

• Synonym(s): L-Lysine homopolymer hydrobromide
• CAS Number: 25988-63-0
• Número MDL: MFCD00237230
• eCl@ss: 32160406
• ID de la sustancia en PubChem: 24898886
• NACRES: NA.26

Properties

• formulario: powder
• mol peso: 15,000-30,000 by viscosity
• color: white to off-white
• application(s): cell analysis
• temp. de almacenamiento: −20°C
• SMILES string: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• InChI key: WBSCNDJQPKSPII-KKUMJFAQSA-N

Description

Aplicación

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Acciones bioquímicas o fisiológicas

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Componentes

La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.

Precaución

Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.

Nota de preparación

This product has a molecular weight of 15,000-30,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Nota de análisis

Molecular weight based on viscosity and was also assayed by MALLS.

Safety Information

• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)