Merck
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P8125

Sigma-Aldrich

Peroxidase from horseradish

Type I, essentially salt-free, lyophilized powder, ≥50 units/mg solid (using pyrogallol)

Synonym(s):
Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase
CAS Number:
Enzyme Commission number:
Número de EC:
Número MDL:
eCl@ss:
32160410
NACRES:
NA.54

tpo

Type I

Nivel de calidad

200

formulario

essentially salt-free, lyophilized powder

specific activity

≥50 units/mg solid (using pyrogallol)

mol peso

~44 kDa

solubilidad

0.1 M phosphate buffer: soluble (pH 6.0)
H2O: soluble

cociente de absorbancia

RZ ≥1.0

temp. de almacenamiento

2-8°C

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Descripción general

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Aplicación

Peroxidase from horseradish has been used:
  • in fecal starch analysis
  • to measure plasma glucose levels by Trinder method
  • to incubate feruloylated arabinoxylan fractions and also to evaluate oxidative coupling
  • in the detection of glycoproteins

Envase

5000, 25000, 50000, 100000, 200000 units in glass bottle

Acciones bioquímicas o fisiológicas

HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase.Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, Pb2+ ions are known to inhibit the enzyme activity.
When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification.

Definición de unidad

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Nota de análisis

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Otras notas

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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