P9029

Sigma-Aldrich

o-Phenylenediamine

Peroxidase substrate, ≥98.0%, powder

Sinónimos:
1,2-Diaminobenzene, OPD, 1,2-Phenylenediamine
Empirical Formula (Hill Notation):
C6H8N2
Número de CAS:
Peso molecular:
108.14
Beilstein/REAXYS Number:
606074
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

vapor density

3.7 (vs air)

vapor pressure

0.01 mmHg ( 25 °C)

assay

≥98.0%

form

powder

color

white to off-white

bp

256-258 °C

mp

98-102 °C

solubility

ethanol: 50 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 337 nm; λem 417 nm (Pyruvic acid derivative)

storage temp.

2-8°C

SMILES string

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

InChI key

GEYOCULIXLDCMW-UHFFFAOYSA-N

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General description

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification. It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.

Packaging

50, 100 g in glass bottle

Caution

May darken in storage.

signalword

Danger

hazcat

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

Flash Point F

276.8 °F - closed cup

Flash Point C

136 °C - closed cup

Certificado de Análisis

Certificado de origen

Bothrops asper envenoming in cattle: Clinical features and management using equine-derived whole IgG antivenom
C.Rodriguez, et al.
The Veterinary Journal, 207(, pages=), 160-163 (2016)
Development of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenom
Navarro D, et al.
Toxicon, 120(3), 1-8 (2016)
Kelsey E Noll et al.
Cell reports, 31(4), 107587-107587 (2020-04-30)
Host genetic factors play a fundamental role in regulating humoral immunity to viral infection, including influenza A virus (IAV). Here, we utilize the Collaborative Cross (CC), a mouse genetic reference population, to study genetic regulation of variation in antibody response...
Expression of hyaluronan synthase 2 or hyaluronidase 1 differentially affect the growth rate of transplantable colon carcinoma cell tumors
Jacobson A, et al.
International Journal of Cancer. Journal International Du Cancer, 102(3), 212-219 (2002)
Mehul S Suthar et al.
Cell reports. Medicine, 1(3), 100040-100040 (2020-08-25)
SARS-CoV-2, the virus responsible for COVID-19, is causing a devastating worldwide pandemic, and there is a pressing need to understand the development, specificity, and neutralizing potency of humoral immune responses during acute infection. We report a cross-sectional study of antibody...
Artículos
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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