RDD002

HEPES

anhydrous, free-flowing, Redi-Dri^™, ≥99.5%

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• Número de EC: 230-907-9
• Número MDL: MFCD00006158
• ID de la sustancia en PubChem: 329824215
• NACRES: NA.25

Properties

• grado: anhydrous
• Nivel de calidad: 100
• Línea del producto: Redi-Dri^™
• ensayo: ≥99.5%
• formulario: crystalline powder
• calidad: free-flowing
• pH: 5.0-6.5 (25 °C, 238 g/L)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubilidad: water: 500 mg/mL, clear, colorless
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used:

• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
• As a component in intracellular solution for the preparation of brain-slice recording
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
• To maintain the pH at 7 in thermal denaturation assay of purified protein
• In isocratic elution of protein using gel filtration
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Información legal

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Código de clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 1
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable