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RDD002

Sigma-Aldrich

HEPES

anhydrous, free-flowing, Redi-Dri, ≥99.5%

Sinónimos:
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O4S
Número de CAS:
Peso molecular:
238.30
Beilstein:
883043
Número de EC:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

100

grado

anhydrous

línea de producto

Redi-Dri

ensayo

≥99.5%

formulario

crystalline powder

calidad

free-flowing

pH

5.0-6.5 (25 °C, 238 g/L)

intervalo de pH útil

6.8 - 8.2

pKa (25 °C)

7.5

solubilidad

water: 500 mg/mL, clear, colorless

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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Descripción general

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Aplicación

HEPES has been used:
  • In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
  • As a component in intracellular solution for the preparation of brain-slice recording
  • As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
  • To maintain the pH at 7 in thermal denaturation assay of purified protein
  • In isocratic elution of protein using gel filtration
  • Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Envase

2.5 kg in poly drum
1 kg in poly bottle

Información legal

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

Cell-free Generation of COPII-coated Procollagen I Carriers.
Yuan L, et al.
Bio-protocol, 7(22) (2017)
Discovery and characterization of a prevalent human gut bacterial enzyme sufficient for the inactivation of a family of plant toxins.
Koppel N, et al.
eLife, 7, e33953-e33953 (2018)
Optogenetic Mapping of Synaptic Connections in Mouse Brain Slices to Define the Functional Connectome of Identified Neuronal Populations.
Mingote S, et al.
The Journal of Neuroscience (2015)
Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations.
Kirkland NT, et al.
Acta Biomaterialia, 8(3), 925-936 (2012)
Hydrogen ion buffers for biological research.
Good NE, et al.
Biochemistry, 5(2), 467-477 (1966)

Artículos

Redi-Dri™ – The World of Free-flowing Hygroscopic Products

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