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RNB400

Sigma-Aldrich

GenElute FFPE RNA Purification Kit

greener alternative
NACRES:
NA.52

características de los productos alternativos más sostenibles

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

categoría alternativa más sostenible

Aligned

enviado en

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Sigma′s GenElute FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Sigma′s GenElute FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including qRT-PCR, reverse transcription PCR, primer extension, expression array assays, and microarray analyses.

  • Extract total RNA (including microRNA) from FFPE samples
  • No phenol extraction step
  • Includes DNase for optional on-column DNA removal
  • Isolated RNA is of the highest quality and integrity

Aplicación

GenElute Water RNA/DNA Purification Kit - 0.45 μm has been used:
  • in total RNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue sections
  • to extract total RNA from paraffin-embedded non-neoplastic lymphatic tissue and neoplastic lymph node tissues
  • to extract total RNA

  • Bioanalyzer
  • Quantitative, real-time RT-PCR for large RNA and small RNA including miRNA
  • RT-PCR for large RNA and small RNA including miRNA
  • Northern blotting
  • RNase protection
  • Primer extension
  • Expression array assays
  • Next Generation Sequencing
  • microRNA Cloning
  • miRNA array
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR

Características y beneficios

  • High quality and integrity of the isolated RNA - The purified total RNA is of the highest quality and integrity, and can be used in any sensitive downstream applications.
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
  • High yields - GenElute′s FFPE RNA Purification Kit allows for the purification of high yields of total RNA.
  • No phenol:chloroform extractions - Total RNA is isolated from FFPE tissue samples without the use of harmful chemicals such as phenol or chloroform.
  • Rapid procedure - Isolate total RNA from FFPE tissue sections using a rapid spin column format in as little as 1 hour

Definición de unidad

Preps

Otras notas

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Información legal

GenElute is a trademark of Sigma-Aldrich Co. LLC

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1C

Riesgos supl.

Código de clase de almacenamiento

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificado de Análisis

Certificado de origen

Role of Kruppel-like factor 4 and heat shock protein 27 in cancer of the larynx
Karam J, et al.
Molecular and Clinical Oncology, 7(5), 808-814 (2017)
AID and TET2 co-operation modulates FANCA expression by active demethylation in diffuse large B cell lymphoma
Jiao J, et al.
Clinical and Experimental Immunology (2018)
Value of serum galectin-3 and midkine level determination for assessing tumor severity in patients with thyroid cancer
Liu YQ
Journal of Hainan Medical College, 23(3), 148-152 (2017)
Jihad Karam et al.
Molecular and clinical oncology, 7(5), 808-814 (2017-11-29)
Late detection and lack of standard treatment strategies in larynx cancer patients result in high levels of mortality and poor prognosis. Prognostic stratification of larynx cancer patients based on molecular prognostic tumor biomarkers may lead to more efficient clinical management.
J Jiao et al.
Clinical and experimental immunology, 195(2), 190-201 (2018-10-26)
Diffuse large B cell lymphoma (DLBCL) is traced to a mature B malignance carrying abnormal activation-induced cytidine deaminase (AID) expression. AID activity initially focuses on deamination of cytidine to uracil to generate somatic hypermutation and class-switch recombination of the immunoglobulin

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