Merck

S0389

Sigma-Aldrich

Sacarosa

for molecular biology, ≥99.5% (GC)

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Sinónimos:
α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopiranosil β-D-fructofuranósido, ββ-D-Fructofuranosil-α-D-glucopiranósido, D(+)-Sacarosa, Azúcar
Empirical Formula (Hill Notation):
C12H22O11
Número de CAS:
Peso molecular:
342.30
Beilstein:
90825
Número CE:
Número MDL:
ID de la sustancia en PubChem:
NACRES:
NA.31

origen biológico

sugar cane

Nivel de calidad

grado

for molecular biology

Análisis

≥99.5% (GC)

formulario

crystals

technique(s)

HPLC: suitable
cryopreservation: suitable
electron microscopy: suitable
enzyme immunoassay: suitable
gas chromatography (GC): suitable
immunohistochemistry: suitable

impurezas

≤0.1% free glucose

color

white

useful pH range

5.5-7 (25 °C, 342 g/L)

mp

185-187 °C (lit.)

solubilidad

H2O: soluble 50 g + 50 mL

trazas de catión

heavy metals (as Pb): ≤5 ppm

absorción

≤0.15 at 280 at 50%
≤0.20 at 260 at 50%

idoneidad

suitable for molecular biology

application(s)

agriculture
agriculture
life science and biopharma
metabolomics
sample preservation

actividad extraña

DNase, RNase, protease, none detected

temp. de almacenamiento

room temp

SMILES string

OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1

InChI key

CZMRCDWAGMRECN-UGDNZRGBSA-N

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Este artículo
S7903S5390S8501
Sucrose for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

S0389

Sucrose

-
Sucrose BioXtra, ≥99.5% (GC)

Sigma-Aldrich

S7903

Sucrose

Premium Grade
Sucrose ≥99% (GC), Grade I, suitable for plant cell culture

Sigma-Aldrich

S5390

Sucrose

-
Sucrose ≥99.5%

Sigma-Aldrich

S8501

Sucrose

-
grade

for molecular biology

grade

-

grade

-

grade

-

assay

≥99.5% (GC)

assay

≥99.5% (GC)

assay

≥99% (GC)

assay

≥99.5%

form

crystals

form

crystals

form

crystals

form

powder

technique(s)

HPLC: suitable, cryopreservation: suitable, electron microscopy: suitable, enzyme immunoassay: suitable, gas chromatography (GC): suitable, immunohistochemistry: suitable

technique(s)

gas chromatography (GC): suitable

technique(s)

cell culture | plant: suitable

technique(s)

gas chromatography (GC): suitable

impurities

≤0.1% free glucose

impurities

Insoluble matter, passes filter test

impurities

≤5 ppm heavy metals (as lead)

impurities

-

Descripción general

Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.

Aplicación

Sucrose has been used:
  • to perform cryoprotection for kidney tissues
  • as a component in the solid germination medium to germinate pollen grains
  • in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
  • in the cryoprotection of fixed brain tissues for immunohistochemistry

Acciones bioquímicas o fisiológicas

Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.

Características y beneficios

  • Ideal for Molecular Biology, Cell Biology and Biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Tested for DNase, RNase, and proteases
  • Molecular biology grade sucrose, sourced from sugar cane

Otras notas

To gain a comprehensive understanding of our extensive range of Disaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Las referencias de los producto se combinan con los tamaños de envase y las cantidades cuando se muestran en la página web, por ejemplo: T1503-25G. Asegúrese de introducir el número de producto ÚNICAMENTE en el campo del Número de producto (ejemplo: T1503).

Ejemplo

T1503
Referencia del producto
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25G
Tamaño del envase/cantidad

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1000309185

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Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch».

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Protocolos

Protocol for the preparation of Antibody Sensitized Sheep Erythrocytes. Includes product numbers and links.

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