The particle size of the powder has not been measured.
S5761
Bicarbonato de sodio
powder, BioReagent, Molecular Biology, suitable for cell culture, suitable for insect cell culture
Sodium bicarbonate
Sinónimos:
Sodium hydrogen carbonate, soda bicarbonate
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500 G
MXP 1,460.00
1 KG
MXP 2,661.00
5 KG
MXP 8,758.00
MXP 1,460.00
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Fórmula lineal:
NaHCO3
Número CAS:
Peso molecular:
84.01
Beilstein/REAXYS Number:
4153970
EC Number:
MDL number:
UNSPSC Code:
12161702
PubChem Substance ID:
NACRES:
NA.75
Assay:
99.5-100.5%
Grade:
Molecular Biology
Form:
powder
Solubility:
water: soluble 100 mg/mL
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Molecular Biology
Quality Level
product line
BioReagent
assay
99.5-100.5%
form
powder
quality
300
technique(s)
cell culture | insect: suitable
cell culture | mammalian: suitable
pH
6.8-7.4
pKa (25 °C)
(1) 6.37, (2) 10.25 (carbonic acid)
solubility
water: soluble 100 mg/mL
density
2.16 g/mL at 25 °C (lit.)
SMILES string
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
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Este artículo | S8875 | S6014 | S8761 |
|---|---|---|---|
| form powder | form powder | form powder | form solution (7.5%) |
| assay 99.5-100.5% | assay ≥99.5% | assay ≥99.7%, 99.7-100.3% | assay - |
| solubility water: soluble 100 mg/mL | solubility - | solubility - | solubility - |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level 400 |
| grade for molecular biology | grade - | grade ACS reagent | grade - |
| pH 6.8-7.4 | pH - | pH - | pH 7.8-8.2 |
General description
Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.
Application
Sodium bicarbonate has been used:
- as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)[1]
- as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells[2]
- as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells[3]
- as a component of medium used for cell culture of human embryonic kidney cells[4]
- as a component of Beltsville thawing solution used for the dilution of semen[5]
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Clase de almacenamiento
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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E Meyer et al.
The European journal of neuroscience, 11(3), 1105-1108 (1999-04-02)
The human slow poke (hSlo) K+ channel was tagged with GFP (green fluorescent protein) at the N-terminus of its alpha-subunit. The fusion protein was expressed transiently in HEK293 cells; it formed functional voltage-gated channels as shown by whole cell patch-clamp
L Keskintepe et al.
Biology of reproduction, 55(2), 333-339 (1996-08-01)
The objective was to establish an in vitro system in which bovine oocytes can be matured, fertilized, and cultured up to the blastocyst stage without support of serum, BSA, or somatic cells. Media consisted of modified tissue culture medium 199
K Okuda et al.
Biology of reproduction, 61(4), 1017-1022 (1999-09-24)
There is sufficient evidence to prove that tumor necrosis factor alpha (TNFalpha) modulates bovine corpus luteum (CL) function. Our previous study demonstrated that functional TNFalpha receptors are present on luteal cells in bovine CL throughout the estrous cycle. The purpose
M Solís et al.
Research in veterinary science, 83(3), 403-409 (2007-03-21)
Porcine rubulavirus (PoRV), also known as blue eye disease (BED) of swine, causes respiratory and reproductive problems in pigs at several developmental stages. To study the effect of PoRV infection on semen production, five boars were infected with 1 x
Chang-Hwan Park et al.
Methods in molecular biology (Clifton, N.J.), 407, 311-322 (2008-05-06)
In this chapter, we introduce a co-culture protocol for human embryonic stem (hES) cell differentiation in which dopamine (DA) neurons with midbrain-specific markers are efficiently derived. Human ES cells on a feeder layer of stromal cells are induced to differentiate
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What is the shelf life after delivery and opening? Is there usually an impact on quality after 2 years? The color of the medium changed and the pH decreased. Does the solubility or function of sodium bicarbonate decrease as it gets older?
1 answer-
This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 5 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
After opening, various factors may influence the performance of the product. Moisture uptake and cross-contamination are common issues that are dependent on product handling and storage. If the product you are using is experience stability issues before the recommended restest date, this reporting should be investigated by our Technical Service team who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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