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Merck

S9626

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

Sinónimos:

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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5000 UNITS

MXP 1,450.00

10000 UNITS

MXP 1,687.00

100000 UNITS

MXP 10,451.00

MXP 1,450.00


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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-772-1
MDL number:
Número CE:

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Nombre del producto

Sulfatase from Helix pomatia, Type H-1, sulfatase ≥10,000 units/g solid

type

Type H-1

form

powder

sulfatase activity

≥10,000 units/g solid

secondary activity

≥300 unit/mg solid β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Quality Level

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Este artículo
S9751SRE0020S8504
application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

-

form

powder

form

aqueous solution

form

lyophilized powder

form

essentially salt-free, lyophilized powder

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

~85 kDa by gel filtration

mol wt

~85 kDa by gel filtration

mol wt

-

mol wt

-

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

100

Application

Sulfatase from Helix pomatia has been used in:
  • sample extraction[1]
  • sulfatase assays[2]
  • enzymatic hydrolysis[3]
  • extract preparation for glucosinolate analysis[4]

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates.[5] It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.[6]

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

used together

Referencia del producto
Descripción
Precios

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)


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Stephen S Hecht et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 13(6), 997-1004 (2004-06-09)
Vegetable consumption, including cruciferous vegetables, is protective against lung cancer, but the mechanisms are poorly understood. The purpose of this study was to investigate the effects of cruciferous vegetable consumption on the metabolism of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)
Ivica Blažević et al.
Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.
Weir K M, et al.
Applied and Environmental Microbiology, 72(5), 3524-3530 (2006)
Glucosinolate enhancement in cabbage induced by jasmonic acid application.
Fritz, V. A., Justen, V. L., Bode, A. M., Schuster, T., & Wang, M.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 45, 1188-1191 (2010)
Use of diabetic test kits to assess the recovery of glucosinolates during isolation of canola protein
Ser W Y, et al.
Food Sci. Technol., 41(5), 934-941 (2008)

Protocolos

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Questions

1–6 of 6 Questions  
  1. How long will Product S9626 retain enzymatic activity when stored at -20C?

    1 answer
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment. For more information, you may access the Product Dating Information document under ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  2. Can Sulfatase S9626 be dissolved in 0.2% NaCl?

    1 answer
    1. Solubility in 0.2% NaCl has not been tested for this product.

      Helpful?

  3. Does Product S9626, Sufatase from Helix pomatia, contain B-Glucuronidase activity?

    1 answer
    1. This product contains >300 units/mg solid B-Glucuronidase activity.

      Helpful?

  4. How is Product S9626, Sulfatase from helix pomatia, prepared?

    1 answer
    1. This enzyme is isolated by acetone fractionation.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is the solubility of Product S9626, Sulfatase from Helix pomatia?

    1 answer
    1. Prior to determining the activity of this enzyme, Sigma reconstitutes it in 0.2% (w/v) sodium chloride.

      Helpful?

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