SAB4200182

Anti-Glutathione Reductase antibody, Mouse monoclonal

clone GR6, purified from hybridoma cell culture

• Synonym(s): Anti-GR, Anti-GSR, Anti-Glutathione-disulfide reductase, Anti-NADPH:oxidized glutathione oxidoreductase
• MDL number: MFCD01323367
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: GR6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~55 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using whole extracts of human HepG2 cells.
• isotype: IgG1
• UniProt accession no.: P00390
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... GSR(2936)

Description

General description

Monoclonal Anti-Glutathione Reductase (mouse IgG1 isotype) is derived from the hybridoma GR6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human Glutathione reductase recombinant protein. Glutathione reductase is a homodimeric enzyme containing one FAD molecule and NADPH binding domain per subunit. This gene is located on human chromosome 8p.

Immunogen

mice immunized with a human Glutathione reductase recombinant protein. The corresponding proteins in rat and mouse share 86% and 85% homology, respectively.

Application

Monoclonal Anti-Glutathione Reductase antibody produced in mouse has been used in:

• immunohistochemical
• immunofluorescence studies
• immunoblotting
• immunoprecipitation

Biochem/physiol Actions

Glutathione reductase (GR) is a ubiquitous flavoenzyme involved in protecting cells from stress. GR catalyses the reduction of oxidized glutathione (GSSG) to glutathione (GSH). It is an essential component of the glutathione redox cycle, which maintains adequate levels of reduced cellular GSH. GSH serves as an antioxidant and also as an electron donor for glutathione peroxidases and a substrate for glutathione-S-transferases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable