SAB4200237

Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal

clone 2H3-D10, purified from hybridoma cell culture

• Synonym(s): Mouse Anti-GST Tag
• MDL number: MFCD02253002
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2H3-D10, monoclonal
• form: buffered aqueous solution
• concentration: ~1.0 mg/mL
• technique(s): western blot: 0.2-0.4 μg/mL using detection limit for GST-fustion protein is ∼ 10ng/lane
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Anti-Glutathione-S-transferase (GST) antibody, mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein. GST is a Phase II metabolic enzyme, expressed ubiquitously in both eukaryotes and prokaryotes. GSTs are classified in to three types based on their cellular localization, namely cytosolic GSTs, mitochondrial GSTs and microsomal GSTs.

Specificity

Monoclonal Anti- Glutathione-S-transferase (GST) recognizes GST from Schistosoma japonicum in GSTfusion proteins.

Immunogen

hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein

Application

Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal has been used in immunoblotting.

Biochem/physiol Actions

Glutathione-S-Transferase (GST) is an enzyme, which catalyzes the reaction of glutathione with a wide range of electrophilic substrates and therefore, plays a role in the detoxification of potential alkylating agents.(5) GST protects the cell against oxidative stress and several toxic molecules. It facilitates the synthesis and modification of leukotrienes and prostaglandins. GST from Schistosoma japonicum, has been cloned in several expression vectors as a 27.5 kDa molecule and used as a tag for expressing proteins of interest. These proteins are thus expressed as GST-fusion proteins. Antibodies to GST are useful in identifying the successful expression of these fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at −20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable