Merck

SAB4200310

Sigma-Aldrich

Anti-Granulin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Sinónimos:
Anti-Acrogranin, Anti-Epithelin, Anti-GEP, Anti-GP88, Anti-GRN, Anti-PCDGF, Anti-PEPI, Anti-PGRN, Anti-Progranulin
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 10-20 μg/mL using A431 cells
western blot: 1-2 μg/mL using MCF7 cell extracts

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GRN(2896)

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Este artículo
S5322SAB4200117F4055
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

technique(s)

indirect immunofluorescence: 10-20 μg/mL using A431 cells, western blot: 1-2 μg/mL using MCF7 cell extracts

technique(s)

immunoprecipitation (IP): 5-10 μg using extracts of Jurkat cells, indirect immunofluorescence: 10-20 μg/mL using human U-2-OS cells, western blot: 1-2 μg/mL using whole extracts of Jurkat cells

technique(s)

indirect immunofluorescence: 5-10 μg/mL using NIH3T3 cells, western blot: 1.5-3.0 μg/mL using A431 cell extract

technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cells lysate, indirect immunofluorescence: 2-5 μg/mL using methanol-acetone fixed heat-shocked NIH3T3 cells, western blot: 1-2 μg/mL using HEK-293T cell lysate, western blot: 2-4 μg/mL using RAT1 cell lysate

Gene Information

human ... GRN(2896)

Gene Information

human ... SIRT1(23411)
mouse ... Sirt1(93759)
rat ... Sirt1(309757)

Gene Information

human ... CORO1C(23603)
mouse ... Coro1c(23790)
rat ... Coro1c(501841)

Gene Information

human ... FMR1(2332)
mouse ... Fmr1(14265)
rat ... Fmr1(24948)

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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General description

Granulin is composed of 7.5 repeat units of a unique, structurally defined cysteine-rich granulin-eptihelin motif.
Granulins (also known as granulin/epithelin precursor [GEP]) are a secreted growth factors expressed mainly by granulocytes. They are secreted as high molecular weight precursor proteins, pro-granulins (PGRN), with seven and a half repeats of 12-cysteine granulin motif. Five human granulins have been isolated and they have a common precursor. PGRN is highly expressed in rapidly proliferating epithelial cells such as keratinocytes. It is also synthesised in neurons of cerebral cortex, hippocampus and cerebellum, central and peripheral nervous system.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human granulin precursor, conjugated to KLH.

Application

Anti-Granulin (C-terminal) antibody produced in rabbit has been used in immunoblotting, immunofluorescence and western blotting.

Biochem/physiol Actions

Granulins are potent mitogenic factors, and mediate proliferation, motility, wound healing and anchorage-dependent growth of fibroblasts. Some of the pathways that are associated with PGRN are MAPK and PI3K/Akt.
Progranulin gene is mitotically quiescent epithelia and is expressed in low levels. It stimulates the proliferation in epithelial cells and promotes their anchorage-independent growth. Overexpression of progranulin confers epithelial invasiveness and tumorigenicity. There is growing evidence that granulin is involved in embryonic and neonatal development, placenta, the epidermis, vasculature angiogenesis, and the developing nervous system. Granulin stimulates an increase in cyclins D1 and cyclins B. Granulin overexpression has been associated with many cancers, including glioblastoma, breast, renal and invasive ovarian cancers. Granulin is highly expressed in motor neurons, it regulates neurite outgrowth and promotes neuronal cell survival. Progranulin gene mutations and PGRN gene haploinsufficiency have been recently proposed to cause a form of frontotemporal dementia (FTLD). It is also associated with the formation of pathogenic Tar-DNA Binding Protein 43 (TDP-43) and ubiquitin positive inclusions.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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A Bateman et al.
The Journal of endocrinology, 158(2), 145-151 (1998-10-15)
The granulin/epithelin motif defines a family of structurally unique proteins, of great evolutionary antiquity, which have been implicated as regulators of cell growth. Recurrent in granulin research are the surprising parallels between the granulin and EGF systems. Both are cysteinerich
Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients
Valdez C, et al.
Human Molecular Genetics, 26(24), 4861-4872 (2017)
Cellular localization of gene expression for progranulin
Daniel R, et al.
The Journal of Histochemistry and Cytochemistry, 48(7), 999-1009 (2000)
T Zanocco-Marani et al.
Cancer research, 59(20), 5331-5340 (1999-10-28)
Growth-regulated cells, such as 3T3 mouse embryo fibroblasts (MEFs), require more than one growth factor for growth, usually the insulin-like growth factor I (IGF-I) in combination with either platelet-derived growth factor or epidermal growth factor. Singly, these growth factors cannot
Progranulin is expressed within motor neurons and promotes neuronal cell survival
Ryan CL, et al.
BMC Neuroscience, 10(1), 130-130 (2009)

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