Merck

SAB4200365

Sigma-Aldrich

Anti-PIWIL1 antibody, Mouse monoclonal

clone PIWIL1-2, purified from hybridoma cell culture

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Sinónimos:
Monoclonal Anti-PIWIL1 antibody produced in mouse, Monoclonal Anti-HIWI, Monoclonal Anti-MIWI, Monoclonal Anti-PIWI, Monoclonal Anti-piwi-like 1
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

antibody product type

primary antibodies

clon

PIWIL1-2, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~95 kDa

species reactivity

human, mouse

concentración

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin® Peroxidase staining system.
immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.

isotipo

IgG2b

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

Gene Information

human ... PIWIL1(9271)
mouse ... Piwil1(57749)

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Este artículo
SAB4200503SAB3500578SAB4200473
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

clone

PIWIL1-2, monoclonal

clone

1L4, monoclonal

clone

polyclonal

clone

4A7, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen ~95 kDa

mol wt

antigen ~60 kDa

mol wt

-

mol wt

antigen ~40 kDa

Descripción general

Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.

Inmunógeno

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:
  • immunofluorescence
  • immunoprecipitation
  • flow cytometry analysis29}
  • immunoblotting
  • immunohistochemistry

Acciones bioquímicas o fisiológicas

Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Información legal

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable


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The biogenesis and function of PIWI proteins and piRNAs: progress and prospect
Thomson T and Lin H
Annual Review of Cell and Developmental Biology, 25, 355-376 (2009)
An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes
He X, et al.
Nucleic Acids Research, 43(7), 3712-3725 (2015)
Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas
Qiao D, et al.
Oncogene, 21(25), 3988-3988 (2002)
High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens
Baumann B, et al.
Prostate, 79(8), 840-855 (2019)
Xue Zhang et al.
Journal of immunology (Baltimore, Md. : 1950), 196(4), 1591-1603 (2016-01-13)
The discovery of PIWI-interacting RNAs (piRNAs) revealed the complexity of the RNA world. Although piRNAs were first deemed to be germline specific, substantial evidence shows their various roles in somatic cells; however, their function in highly differentiated immune cells remains

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