SAB4200369

Anti-Nectin-2/PVRL2 antibody, Mouse monoclonal

clone R2.5254.2, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse, Anti-CD112, Anti-HVEB, Anti-PRR2, Anti-PVRL2, Anti-PVRR2, Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: R2.5254.2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: rat, human, monkey, mouse
• concentration: ~1.0 mg/mL
• technique(s): flow cytometry: 5-10 μg/test using using 1x10⁶ MDA-MB-231 cells; immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells; indirect ELISA: suitable; western blot: suitable
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... NECTIN2(5819) , PVRL2(5819) , PVRL2(308417) ; mouse ... Nectin2(19294)

Description

General description

Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.

Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.

Specificity

The antibody has been reported to block cell adhesion and herpes virus entry.

Immunogen

3T3 cells expressing human Nectin-2.

Application

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• flow cytometry

Biochem/physiol Actions

Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable