Merck

SAB4200522

Sigma-Aldrich

Anti-IDH1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

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Sinónimos:
Anti-IDP, Anti-PICD, Anti-isocitrate dehydrogenase 1 (NADP+), soluble, IDH
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~45 kDa

species reactivity

rat, mouse, human

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 20 μg/mL using human kidney.
indirect immunofluorescence: 1-2 μg/mL using SHSY5Y cells.
western blot: 1-2 μg/mL using extracts of NIH3T3 cells and of rat cerebellum (S1 fraction).

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IDH1(3417)
mouse ... Idh1(15926)
rat ... Idh1(24479)

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Este artículo
SAB4200592SAB3500511SAB5701050
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

liquid

Gene Information

human ... IDH1(3417)
mouse ... Idh1(15926)
rat ... Idh1(24479)

Gene Information

human ... IDH1(3417)

Gene Information

human ... IDH1(3417)

Gene Information

human ... IDH1(3417)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

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General description

IDH1 is a cytoplasmic nicotinamide adenine dinucleotide phosphate (NADP+)-dependent enzyme, localized both in the cytoplasm and peroxisomes. The IDH1 gene is mapped to human chromosome 2q34. It comprises a tripeptide peroxisome targeting signal 1 in the C-terminal region and is majorly expressed in the liver.

Specificity

Anti-IDH1 (C-terminal)specifically recognizes human, rat and mouse IDH1.

Immunogen

synthetic peptide corresponding to a C-terminal sequence of human IDH1, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse IDH1.

Application

Anti-IDH1 (C-terminal) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) is a key metabolic enzyme that catalyzes the oxidative decarboxylation of isocitrate into α-ketoglutarate (αKG) utilizing either nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+) as co-substrates. Although the function and structure of IDH1 have been well characterized, it has only recently been implicated in cancer. IDH1 appears to have a tumor suppressor activity and its inactivation contributes to tumorigenesis partially mediated by induction of the hypoxia-inducible factor 1α (HIF1) pathway. A genome-wide mutation study has shown that IDH1 is mutated in glioblastoma, acute myeloid leukemia (AML) and chondrosarcoma. Mutations in IDH1 specific to Arg132 (R132) impart the enzyme the ability to generate 2-hydroxyglutarate (2HG) instead of αKG. Elevated levels of 2HG are correlated with increased risk of malignant brain tumors, and block cell differentiation by impairing histone demethylation. Several IDH1 mutations have been identified in gliomas, including R132H, R132C, R132S, R132G and R132L, each may result in different tumor type with varied malignant progression.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, storeat 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Optional

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Isocitrate dehydrogenase 1 and 2 mutations in cancer: alterations at a crossroads of cellular metabolism
Reitman ZJ and Yan H
Journal of the National Cancer Institute, 102(13), 932-941 (2010)
IDH mutation impairs histone demethylation and results in a block to cell differentiation
Lu C, et al.
Nature, 483(7390), 474-478 (2012)
Molecular pathogenesis of IDH mutations in gliomas
Ichimura Koichi
Brain Tumor Pathology, 29(3), 131-139 (2012)

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