Merck

SAB4200716

Sigma-Aldrich

Anti-Vimentin antibody, Mouse monoclonal

enhanced validation

clone VIM-13.2, purified from hybridoma cell culture

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Sinónimos:
Anti-F5H288
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified from hybridoma cell culture

antibody product type

primary antibodies

clon

VIM-13.2, monoclonal

formulario

buffered aqueous solution

mol peso

~58 kDa

species reactivity

mouse, bovine, canine, rat, monkey, human, chicken

validación mejorada

independent
Learn more about Antibody Enhanced Validation

concentración

~1.0 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract
immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells
immunohistochemistry: suitable

isotipo

IgM

enviado en

dry ice

temp. de almacenamiento

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Vim(280955)
chicken ... Vim(420519)
dog ... Vim(477991)
human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)
rhesus monkey ... Vim(705289)

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Este artículo
V5255V6389V2258
antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

clone

VIM-13.2, monoclonal

clone

VIM-13.2, monoclonal

clone

V9, monoclonal

clone

LN-6, monoclonal

form

buffered aqueous solution

form

-

form

buffered aqueous solution

form

-

mol wt

~58 kDa

mol wt

-

mol wt

antigen ~58 kDa

mol wt

-

species reactivity

mouse, bovine, canine, rat, monkey, human, chicken

species reactivity

chicken, rat, human, rabbit, frog, mouse

species reactivity

hamster, monkey, dog, horse, bovine, rat, gerbil, chicken, pig, feline, human

species reactivity

pig, mouse, sheep, human, bovine, rabbit, feline, rat

Descripción general

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.

Inmunógeno

human foreskin fibroblast extract

Aplicación

Anti-Vimentin antibody, Mouse monoclonal has been used in:
  • western blotting
  • indirect immunofluorescence
  • immunohistochemistry

Acciones bioquímicas o fisiológicas

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

nwg

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable


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Podoplanin regulates mammary stem cell function and tumorigenesis by potentiating Wntbeta-catenin signaling
Bresson L, et al.
Development, 145(4), dev160382-dev160382 (2018)
Morphological and quantitative analysis on alpha-tubulin modifications in glioblastoma cells
Gadau SD
Neuroscience Letters, 687, 111-118 (2018)
Azariyas A Challa et al.
Molecular and cellular biology, 31(18), 3773-3789 (2011-07-13)
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