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SAE0086

Sigma-Aldrich

Peptidyl Arginine Deiminase Type 4

human recombinant, expressed in E. coli, aqueous solution

Synonym(s):
Protein arginine iminohydrolase 4, PAD4, PADI4
Enzyme Commission number:
3.5.3.15

Nivel de calidad

200

origen biológico

human

recombinante

expressed in E. coli

ensayo

≥90% (SDS-PAGE)

formulario

aqueous solution

specific activity

≥200 units/mg protein

mol peso

74 kDa

Nº de acceso UniProt

application(s)

genomic analysis

enviado en

dry ice

temp. de almacenamiento

−20°C

Gene Information

human ... PADI4(23569)

Descripción general

Peptidyl Arginine Deiminases (PADs) perform post-translational deiminations of proteins. PADs are calcium-dependent enzymes that catalyze the conversion of L-arginine residues to L-citrulline. The catalyzed reaction is as follows:

2+
Ca Protein--[L-arginine] + H2O ––––> Protein--[L-citrulline]

There are five mammalian PADs sub-types, which differ in substrate specificity and tissue distribution. PAD enzymes are highly homologous, with 50–60% sequence similarity.1 PADs play important roles in gene regulation by citrullination of arginine residues on histones H3, H2A, and H4. Overexpression of PADs has been found in conditions such as rheumatoid arthritis, Alzheimer′s disease, multiple sclerosis, lupus,Parkinson′s disease, and cancer.2,3

PAD4 is widely expressed in the immune system with expression pathology in various tumor and cancer cell lines.5 In cells, PAD4 can be found in the cytoplasm and nucleus. PAD4 activity has been correlated to the development and progress of Rheumatoid Arthritis (RA).2,5 A study in mice has indicated a mechanism for the development of RA by anticitrullin autoimmunity.6

This recombinant human PAD4 is expressed in Escherichia coli as a fusion protein. The fusion partner is removed by HRV3C protease digestion, followed by chromatographic purification.

Acciones bioquímicas o fisiológicas

Calcium is required for peptidylarginine deiminase activity in vitro.

Definición de unidad

One unit will produce 1 μmole of N-a-benzoylcitrulline ethyl ester from BAEE per hour at 37 °C at pH 7.7.

Forma física

Supplied as an aqueous solution in 10 mM Tris (pH 7.5), 500 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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