SAE0150

Sigma-Aldrich

Chondroitinase ABC from Proteus vulgaris

recombinant, low endotoxin, aqueous solution, ≥100 U/ml, 50-250 units/mg protein, BSA free

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Quality Level

specific activity

50-250 units/mg protein

shipped in

dry ice

storage temp.

−70°C

General description

Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of Δ 4-unsaturated tetra- and di-saccharides.

Application

  • Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage
  • Neural regeneration research
  • Eliminative degradation of all forms of Chondroitin sulfate: A, B and C
  • Quantitation of glucosaminoglycans (mucopolysaccharides)

Features and Benefits

  • Low Endotoxin
  • BSA Free
  • Recombinant
  • Endolytic component only, does not contain exolytic enzyme.
  • Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate

Components

Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water

Compatibility

pH optimum:
  • pH 8.0 (chondroitin sulfate)
  • pH 6.8 (hyaluronic acid)

Temperature optimum: 37 °C
Activator: 0.05 M acetate 
Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+ 

Unit Definition

1 unit will liberate 1.0 μmole of unsaturated disaccharides per min at pH 8.0 at 37 °C, using chondroitin sulfate from shark cartilage (C4384) as substrate.

Storage and Stability

Store the product at -70 °C. When stored unopened at -70 °C, the buffer is stable for at least two years.

Other Notes

This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.

WGK Germany

WGK 3

Two distinct chondroitin sulfate ABC lyases an endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides
Hamai, A. et al.
The Journal of Biological Chemistry, 9123-9130 (1997)
Functions of Chondroitin Sulfate and Heparan Sulfate in the Developing Brain
Maeda, N., et al.
Neurochemical Research, 1228-1240 (2011)

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