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SCA20

Supelco

Sucrose Assay Kit

sufficient for 20 assays

EC Number:
NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

analyte chemical class(es)

sugars (sucrose)

application(s)

photometry: suitable

Featured Industry

Food and Beverages
General Analytical

storage temp.

2-8°C

General description

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

Solo componentes del kit

Referencia del producto
Descripción

  • glucose assay reagent 50 mL

  • sucrose assay reagent 1 mL/vial

  • sucrose standard 500 mg

Storage Class Code

12 - Non Combustible Liquids

Certificado de Análisis

Certificado de origen

Bergmeyer, H.U. and Bernt, E.
Methods of Enzymatic Analysis, 1176-1176 (1974)
Ching Man Wai et al.
BMC genomics, 18(1), 773-773 (2017-10-13)
Sugarcane is an emerging dual-purpose biofuel crop for energy and sugar production, owing to its rapid growth rate, high sucrose storage in the stems, and high lignocellulosic yield. It has the highest biomass production reaching 1.9 billion tonnes in 2014...
Manish Pandey et al.
Scientific reports, 11(1), 3000-3000 (2021-02-06)
Plant bioregulators (PBRs) represent low-cost chemicals for boosting plant defense, especially under stress conditions. In the present study, redox based PBRs such as thiourea (TU; a non-physiological thiol-based ROS scavenger) and hydrogen peroxide (H2O2; a prevalent biological ROS) were assessed...
Quantitative 1H NMR with external standards: use in preparation of calibration solutions for algal toxins and other natural products.
Burton IW, Quilliam MA, Walter JA.
Analytical Chemistry, 77(10), 3123-3131 (2005)
D. H. Lewis
Storage Carbohydrates in Vascular Plants: Distribution, Physiology and Metabolism, 53 -53 (1984)

Protocolos

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

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