Merck
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SHC005V

Sigma-Aldrich

MISSION® pLKO.1-puro eGFP shRNA Control Transduction Particles

shRNA sequence targeting eGFP

Synonym(s):
MISSION TurboGFP Control Transduction Particles
NACRES:
NA.51

Nivel de calidad

200

línea de producto

MISSION®

concentración

≥1x106 VP/ml (via p24 assay)

technique(s)

capture ELISA: 106 TU/mL using p24

enviado en

dry ice

temp. de almacenamiento

−70°C

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Descripción general

The MISSION eGFP shRNA Control Transduction Particles contain an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761). The eGFP shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing eGFP.
The eGFP shRNA Control Transduction Particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-eGFP shRNA (Prod. No. SHC005). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Aplicación

To see more application data, protocols, vector maps visit sigma.com/shrna.

Información legal

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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