SHPM01

Sigma-Aldrich

MISSION® LentiPlex® Mouse Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

NACRES:
NA.51
En este momento no podemos mostrarle ni los precios ni la disponibilidad

product line

MISSION®

concentration

≥5x108 VP/ml (via p24 assay)

application(s)

capture ELISA: 5 × 108 TU/mL using p24

shipped in

dry ice

storage temp.

−70°C

Categorías relacionadas

General description

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma′s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Mouse shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 75,000 shRNA constructs from the TRC collection targeting 15,000+ mouse genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

The library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Each library is enough material for six screens at 100X representation.

Please visit sigma.com/lentiplex to learn more and see example data and uses.

Other Notes

Deconvolution of shRNA Pools
Sigma′s
deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

  • Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
  • Comprehensive, reproducible results from pooled shRNA screens
  • Statistically robust and information-rich data

MISSION custom pooled viral shRNA libraries

Pooled shRNA RNAi screening approaches are fast becoming an economical alternative for cell based screens. MISSION RNAi is now offering custom pooled shRNA viral libraries. Just give us your list of genes or pick a gene set and leave the rest to us!

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

Legal Information

LentiPlex is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

RIDADR

UN3373 - class 6.2 Biological substance, Category B

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Wei Li et al.
Journal of molecular biology, 430(17), 2734-2746 (2018-05-26)
Xist (inactivated X chromosome specific transcript) is a prototype long noncoding RNA in charge of epigenetic silencing of one X chromosome in each female cell in mammals. In a genetic screen, we identify Mageb3 and its homologs Mageb1 and Mageb2...
Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Lin, Yu-yi, et al.
Nature (2012)
Ting Zhao et al.
Cell stem cell, 23(1), 31-45 (2018-06-26)
Chemical reprogramming provides a powerful platform for exploring the molecular dynamics that lead to pluripotency. Although previous studies have uncovered an intermediate extraembryonic endoderm (XEN)-like state during this process, the molecular underpinnings of pluripotency acquisition remain largely undefined. Here, we...
Richard Possemato et al.
Nature, 476(7360), 346-350 (2011-07-16)
Cancer cells adapt their metabolic processes to drive macromolecular biosynthesis for rapid cell growth and proliferation. RNA interference (RNAi)-based loss-of-function screening has proven powerful for the identification of new and interesting cancer targets, and recent studies have used this technology...
Christine Karlsson et al.
Methods in molecular biology (Clifton, N.J.), 650, 29-43 (2010-08-06)
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells....
Artículos
Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
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