SML0655

Sigma-Aldrich

Shiga toxin 1, B subunit, HIS-tagged

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Sinónimos:
SLT1, STxB, STX1
NACRES:
NA.77
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

lyophilized

suitability

endotoxin tested

shipped in

dry ice

storage temp.

−20°C

Biochem/physiol Actions

The Shiga toxins are a family of related protein toxins secreted by certain types of bacteria. Shiga toxin (Stx) is produced by Shigella dysenteriae; whereas, the Shiga-like toxins, Stx1 and Stx2, with a few known isoforms, are secreted by specific strains of Escherichia coli named Shiga-toxin-producing E. coli (STEC), such as E. coli O157:H7, which causes bloody diarrhea and hemorrhagic colitis in humans, sometimes resulting in fatal systemic complications.

Stx1 is identical to Stx, while the Stx2 isoforms share less sequence similarity with Stx (∼60%) and are immunologically distinct. In spite of the differences in their amino acid sequence, all Stx isoforms share the same overall toxin structure and mechanism of action.

Shiga toxins consists of two polypeptides. An A chain and a B chain non-covalently associate with an apparent stoichiometry of one A and five B chains to form the holotoxin. The catalytic A subunit has
RNA N-glycosidase activity that inhibits eukaryotic protein synthesis. The B subunits form a pentamer, which recognizes and binds to the functional cell-surface receptor globotriaosylceramide Gb3; Gala(1-4)-Galb(1-4)-Glcb1-ceramide. Gb3 is overexpressed in membranes of numerous tumor cells, therefore STxB binding to Gb3 receptors may be useful for cell-specific vectorization, labeling, and imaging purposes.

Physical form

The product is a recombinant Shiga toxin B subunit, which contains 69 amino acid residues and a HIS-tag at the C-terminus. It is lyophilized from 0.2 μm filtered solution of phosphate buffer without any carrier protein.

Preparation Note

Reconstitute the contents of the vial using water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 2-8 °C for up to 4 months or at -20 °C for extended use.

Analysis Note

Gb3 Binding activity: significant binding above background is achieved with ≤1 μg/mL of STxB. The activity of STxB is measured by its ability to bind to Gb3, which requires its pentameric form.

pictograms

Exclamation mark

signalword

Warning

pcodes

Hazard Codes

T

Risk Statement

23/24/25

Safety Statement

36/37-45

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

In-Sun Shin et al.
Chemical & pharmaceutical bulletin, 54(4), 522-527 (2006-04-06)
A two-step binding assay for globotriaosylceramide (Gb3) content was developed by histidine-tagging strategy, which is a well-established method for the purification of recombinant proteins. The complete binding of the recombinant His-tagged Shiga toxin 1B subunit (1B-His) (1 microg/ml) to the...

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