≥98% (HPLC)

DFHBI 1T, (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4H)-one, (5Z)-5-(3,5-Difluoro-4-hydroxyphenyl)methylene-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
Empirical Formula (Hill Notation):
Número de CAS:
Peso molecular:
MDL number:
En este momento no podemos mostrarle ni los precios ni la disponibilidad


≥98% (HPLC)



storage condition

protect from light


white to beige


DMSO: 2 mg/mL, clear

storage temp.


Biochem/physiol Actions

DFHBI-IT is a membrane-permeable, non-cytotoxic, RNA aptamers-activated fluorescence probe widely employed in varous detection systems utilizing compatible RNA tags such as Spinach, Spinach2, iSpinach, and Broccoli. Upon binding Spinach2, DFHBI-1T offers brighter fluorescence than DFHBI due to increased extinction coefficient & quantum yield (by 1.4- and 1.3-fold, respectively). DFHBI-1T is also better suited for devices optimized for GFP or fluorescein detection/imaging due to its red shift in excitation & emission wavelengths (Spinach2-comlex Ex/Em = 482/505 nm with DFHBI-1T vs 447/501 nm with DFHBI).

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Jieun Sim et al.
Chemical communications (Cambridge, England), 55(25), 3618-3621 (2019-03-09)
Here, we present an ultra-enhanced immunoassay for sensitive and reliable biomarker detection using layer-by-layer assembly and transcription-assisted light-up aptamer generation to induce signal amplification. This dendrimer structure-based transcription immunoassay is ∼1500 times more sensitive than commercial fluorescence ELISA, achieving a...
Esther Braselmann et al.
Nature chemical biology, 14(10), 964-971 (2018-08-01)
RNAs directly regulate a vast array of cellular processes, emphasizing the need for robust approaches to fluorescently label and track RNAs in living cells. Here, we develop an RNA imaging platform using the cobalamin riboswitch as an RNA tag and...
Tamaki Endoh et al.
Small (Weinheim an der Bergstrasse, Germany), 15(26), e1805062-e1805062 (2019-02-19)
RNA aptamers are useful building blocks for constructing functional nucleic acid-based nanoarchitectures. The abilities of aptamers to recognize specific ligands have also been utilized for various biotechnological applications. Solution conditions, which can differ depending on the application, impact the affinity...
Wenjiao Song et al.
Journal of the American Chemical Society, 136(4), 1198-1201 (2014-01-08)
Spinach and Spinach2 are RNA aptamers that can be used for the genetic encoding of fluorescent RNA. Spinach2 binds and activates the fluorescence of (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one (DFHBI), allowing the dynamic localizations of Spinach2-tagged RNAs to be imaged in live cells. The...
Alexis Autour et al.
Methods (San Diego, Calif.), 161, 46-53 (2019-03-25)
Biosensors are biological molecules able to detect and report the presence of a target molecule by the emission of a signal. Nucleic acids are particularly appealing for the design of such molecule since their great structural plasticity makes them able...

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