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SRP0116

Sigma-Aldrich

Sirtuin 2 human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym(s):
SIR2L2, SIRT2, sir2-like 2
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

ensayo

≥80% (SDS-PAGE)

formulario

aqueous solution

mol peso

35.5 kDa

envase

pkg of 100 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

concentración

>0.02 mg/mL

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... SIRT2(22933)

Descripción general

Silent information regulator 2 (Sir2) or sirtuin 2 is encoded by the gene mapped to human chromosome 19q13.2. It belongs to class Ib of sirtuin protein family. Sir2 is characterized with a catalytic domain containing two different domains that bind NAD and the acetyl-lysine substrate, respectively. Apart from this, it also constitutes variable NH2 and COOH-terminal domains involved in the regulation of subcellular localizations and catalytic activity. sirtuin 2 is mainly expressed in cytosol but its migration between cytosol and nucleus facilitates the deacetylation of both α-tubulin and histones.
Human Sirtuin 2, GenBank Accession No. NM_030593, amino acids 50-356 with C-terminal His tag, MW = 35.5kDa, expressed in Escherichia coli expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Acciones bioquímicas o fisiológicas

Silent information regulator 2 (Sir2) acts as a nicotine adenine dinucleotide (NAD) dependent deacetylase and is implicated in various biological processes such as regulation of transcriptional repression, recombination, the cell-division cycle, microtubule organization, and cellular responses to DNA-damaging agents. In addition, sirtuins also regulate the process involved in aging. Sir2 plays a vital role in mammalian metabolic homeostasis and is considered to be a potent therapeutic target for the treatment of type 2 diabetes. Sir2 has a crucial role in reduction of microglial activation and brain inflammation. Therefore, loss of Sir2 activity increases the risk of susceptibility to neurodegenerative diseases.

Definición de unidad

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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