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SRP0151

Sigma-Aldrich

SMYD2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):
KMT3C, Lysine N-methyltransferase 3C, SET and MYND domain containing 2, ZMYND14, Zinc finger, MYND domain containing 14
NACRES:
NA.32

origen biológico

human

recombinante

expressed in baculovirus infected insect cells

ensayo

≥80% (SDS-PAGE)

formulario

aqueous solution

mol peso

49.7 kDa

envase

pkg of 20 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

concentración

>0.02 mg/mL

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... SMYD2(56950)

Descripción general

SET and MYND domain containing 2 (SMYD2) is encoded by the gene mapped to human chromosome 1q32.3-q41. The encoded protein belongs to SMYD family of protein lysine methyltransferases.
Human SMYD2 (GenBank Accession No. NM_020197), full length with N-terminal DDDDK tag (FLAG), MW = 49.7kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Aplicación

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Acciones bioquímicas o fisiológicas

SET and MYND domain containing 2 (SMYD2) might act as a putative oncogene by methylating p53 and reducing its tumor suppressive role. Overexpression of SMYD2 gene is associated with pathogenesis and metastasis of oesophageal cancer, squamous cell carcinoma (ESCC). Therefore, it is considered as a potent therapeutic target for ESCC. SMYD2 has capacity to catalyze the methylation of histone and non-histone substrates. AZ505 acts as a potent and selective inhibitor of SMYD2 and aids in the improvement of novel cancer treatment.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Certificate of Analysis

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Certificate of Origin

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