Merck
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SRP0187

Sigma-Aldrich

Bcl-xL Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):
Apoptosis regulator Bcl-X, BCL-XL/S, BCL2L1, Bcl-2-like protein 1
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

ensayo

≥90% (SDS-PAGE)

formulario

aqueous solution

mol peso

28 kDa

envase

pkg of 100 μg

condiciones de almacenamiento

avoid repeated freeze/thaw cycles

concentración

>0.02 mg/mL

Nº de acceso NCBI

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... BCL2L1(598)

Descripción general

BCL2L1 (B-cell lymphoma 2 like 1) gene codes for the anti-apoptotic protein called Bcl-xL (B-cell lymphoma-extra large) and its isoform Bcl-xS. Bcl-xS functions as an antagonist of Bcl-xL. This gene is localized to human chromosome 20q11.21.

Aplicación

Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.

Acciones bioquímicas o fisiológicas

BCL2L1 (B-cell lymphoma 2 like 1) or Bcl-xS functions as a dominant negative regulator of the anti-apoptotic isoform Bcl-xL. Bcl-xL is involved in modulating anti-apoptotic capacity of chemotherapy-resistant tumors. This protein is highly expressed in michigan cancer foundation 7 (MCF7) human breast carcinoma cells, and studies show that overexpression of Bcl-xS in these MCF-7 cells results in heightened sensitivity to chemotherapy-induced apoptosis by agents such as VP-16 or taxol.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificate of Analysis

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Certificate of Origin

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