SRP0189

Sigma-Aldrich

Pyruvate kinase M2 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Sinónimos:
Thyroid hormone-binding protein 1 (THBP1), Pyruvate kinase, muscle, M2-PK tumor, OPA-interacting protein 3 (OIP-3), Pyruvate kinase 3 (PK3)
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

mouse

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

58 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... PKM2(18746)

General description

Pyruvate kinase M2 (PKM2), also known as pyruvate kinase 3 (PK3), is an isoform of pyruvate kinase that is expressed in some differentiated tissues, such as lung, fat, retina, and pancreatic islets, as well as in normal proliferating cells and embryonic cells. PKM2/pk3 is encoded by the gene mapped to mouse chromosome 9.
Murine Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_011099), amino acids 2-end, with N-terminal His tag, MW = 58kDa, expressed in an Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Elevated expression of PKM2 is observed in most of the cancer cells. Hence, this protein can be considered as a potent therapeutic target for cancers. PKM2 plays an important role in the discharge of excessive intracellular reactive oxygen species (ROS), which otherwise damage cellular components and decrease cell viability. Protein kinase activity of PKM2 is involved in the regulation of gene expression and tumorigenesis. PKM2 is an important rate-limiting enzyme involved in final step of glycolysis and it has an essential role in metabolic reprogramming throughout cancer progression.

Unit Definition

One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Certificado de Análisis
PKM2 and cancer: The function of PKM2 beyond glycolysis.
Dong G
Oncology Letters, 11(3), 1980-1986 (2016)
Polymorphism of kidney pyruvate kinase in the mouse is determined by a gene, Pk-3, on chromosome 9.
Peters J
Biochemical Genetics, 19(7-8), 757-769 (1981)
Weiwei Yang et al.
Cancer letters, 339(2), 153-158 (2013-06-25)
Altered metabolism is fundamental to the growth and survival of cancer cells. Pyruvate kinase M2 (PKM2), a key enzyme in cancer metabolism, has been demonstrated to play a central role not only in metabolic reprogramming but also in direct regulation...
Artículos
We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.
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