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SRP0207

Sigma-Aldrich

PEST Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):
Lver regeneration-related protein LRRG084, PEST proteolytic signal containing nuclear protein
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

ensayo

≥90% (SDS-PAGE)

formulario

aqueous solution

mol peso

61.4 kDa

envase

pkg of 20 μg

concentración

>0.02 mg/mL

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... PCNP(57092)

Descripción general

Protein tyrosine phosphatase, Catalytic domain, (amino acids 2-300) (Gene bank accession number NM_002835) with N-terminal GST tag, MW = 61.4 kDa, expressed in an E. coli expression system.

Aplicación

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Definición de unidad

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Certificate of Analysis

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Certificate of Origin

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