SRP0207

PEST Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): Lver regeneration-related protein LRRG084, PEST proteolytic signal containing nuclear protein
• NACRES: NA.32

Properties

• origen biológico: human
• recombinante: expressed in E. coli
• ensayo: ≥90% (SDS-PAGE)
• formulario: aqueous solution
• mol peso: 61.4 kDa
• envase: pkg of 20 μg
• concentración: >0.02 mg/mL
• Nº de acceso NCBI: NM_002835
• Nº de acceso UniProt: Q8WW12
• enviado en: dry ice
• temp. de almacenamiento: −70°C
• Gene Information: human ... PCNP(57092)

Description

Descripción general

Protein tyrosine phosphatase, Catalytic domain, (amino acids 2-300) (Gene bank accession number NM_002835) with N-terminal GST tag, MW = 61.4 kDa, expressed in an E. coli expression system.

Aplicación

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Definición de unidad

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Nota de preparación

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.