SRP0242

Sigma-Aldrich

EGFR (T790M L858R) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Sinónimos:
ERBB1, Proto-oncogene c-ErbB-1, PIG61, HER1, Epidermal growth factor receptor
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥70% (SDS-PAGE)

form

aqueous solution

mol wt

88 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EGFR(1956)

General description

Epidermal growth factor receptor (EGFR) can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor-α (TGF-α), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin and epigen.<The EGFR gene is mapped to human chromosome 7p11.2 and encodes a 170 kDa receptor protein belonging to the HER (Receptor tyrosine-protein kinase erbB-4) family of receptor tyrosine kinases.<

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. The receptor undergoes dimerization and phosphorylation of tyrosine kinase. Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers. Mutation in the EGFR gene is associated with the risk of tumor development including glioma, colorectal adenoma, colorectal, breast and non-small cell lung cancer.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Certificado de Análisis
Effect of epidermal growth factor receptor gene polymorphisms on prognosis in glioma patients.
Li B, et al.
Oncotarget, 7(39), 63054-63054 (2016)
A chromosomal region 7p11.2 transcript map: its development and application to the study of EGFR amplicons in glioblastoma.
Eley GD
Neuro-Oncology, 4(2), 86-94 (2002)
Molecular mechanisms of resistance to the EGFR monoclonal antibody cetuximab.
Brand TM
Cancer Biology & Therapy, 11(9), 777-792 (2011)

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