SRP0278

Sigma-Aldrich

PDE11A4 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Sinónimos:
PPNAD2, cAMP and cGMP cyclic nucleotide phosphodiesterase 11A, Phosphodiesterase 11A4
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

131 kDa

packaging

pkg of 10 μg

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PDE11A(50940)

General description

PDE11A (phosphodiesterase 11A) belongs to the PDE protein family, and is expressed in three isoforms. Its mRNA has been found to be expressed in skeletal muscle, prostate, kidney, liver, pituitary, and salivary glands and testis. All PDEs contain a conserved PDE catalytic domain at the C-terminal, which also contains the PDE signature motif HDX2HX4N. This gene is localized to human chromosome 2q31–2q35.
Human PDE11A4 (GenBank Accession No. BAB62712), full length with N-terminal GST tag, MW = 131kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Phosphodiesterases (PDEs) function as regulators of cyclic nucleotide levels. PDE11A is a dual-specificity PDE that might be involved in controlling both cGMP and cAMP levels under physiological conditions. A genome-wide study shows that the inactivation of this gene is linked with adrenocortical hyperplasia. Polymorphisms in this gene are also linked with ACTH (adrenocorticotropic hormone)-independent macronodular adrenal hyperplasia (AIMAH). PDE11A is also involved in the control of cortisol secretion. In Carney complex (CNC) patients with germline PRKAR1A mutations, polymorphisms in PDE11A might influence the development of testicular and adrenal tumors by acting as a modifier of the phenotype.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 5 mM glutathione and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Certificado de Análisis
A genome-wide scan identifies mutations in the gene encoding phosphodiesterase 11A4 (PDE11A) in individuals with adrenocortical hyperplasia.
Horvath A et al
Nature Genetics, 38(7), 794-800 (2006)
Phosphodiesterase 11A (PDE11A) gene defects in patients with acth-independent macronodular adrenal hyperplasia (AIMAH): functional variants may contribute to genetic susceptibility of bilateral adrenal tumors.
Vezzosi D et al
The Journal of Clinical Endocrinology and Metabolism, 97(11), E2063-E2069 (2012)
Frequent phosphodiesterase 11A gene (PDE11A) defects in patients with Carney complex (CNC) caused by PRKAR1A mutations: PDE11A may contribute to adrenal and testicular tumors in CNC as a modifier of the phenotype.
Libe R et al
The Journal of Clinical Endocrinology and Metabolism, 96(1), E208-E214 (2011)
Molecular cloning and characterization of a distinct human phosphodiesterase gene family: PDE11A.
Fawcett L et al
Proceedings of the National Academy of Sciences of the USA, 97(7), 3702-3707 (2000)

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