All Photos(2)
recombinante
expressed in baculovirus infected Sf9 cells
ensayo
70-95% (SDS-PAGE)
formulario
frozen liquid
specific activity
3.0-5.0 nmol/min·mg (radiometric)
mol peso
~76 kDa
concentración
0.1 μg/μL
nº de acceso
NM_017433
enviado en
dry ice
temp. de almacenamiento
−70°C
Gene Information
human ... MYO3A(53904)
Aplicación
Useful for kinase assay and Western blot.
Acciones bioquímicas o fisiológicas
Recombinant human MYO3alpha (1-434) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3alpha belongs to the myosin superfamily which are members of the large myosin superfamily and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as MYO3alpha, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors (1). MYO3alpha plays an important role in hearing in humans and mutations in the MYO3alpha have been shown to cause nonsyndromic progressive hearing loss (2). MYO3alpha is highly expreesed in retina and cochlea.
Reconstitución
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Almacenamiento y estabilidad
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Información legal
PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
Código de clase de almacenamiento
10 - Combustible liquids
WGK
WGK 1
Punto de inflamabilidad F
Not applicable
Punto de inflamabilidad C
Not applicable
Certificate of Analysis
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Certificate of Origin
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