Merck
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SRP0728

Sigma-Aldrich

MYO3ALPHA, Active

PRECISIO® Kinase, recombinant

recombinante

expressed in baculovirus infected Sf9 cells

ensayo

70-95% (SDS-PAGE)

formulario

frozen liquid

specific activity

3.0-5.0 nmol/min·mg (radiometric)

mol peso

~76 kDa

concentración

0.1 μg/μL

nº de acceso

NM_017433

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... MYO3A(53904)

Aplicación

Useful for kinase assay and Western blot.

Acciones bioquímicas o fisiológicas

Recombinant human MYO3alpha (1-434) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3alpha belongs to the myosin superfamily which are members of the large myosin superfamily and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as MYO3alpha, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors (1). MYO3alpha plays an important role in hearing in humans and mutations in the MYO3alpha have been shown to cause nonsyndromic progressive hearing loss (2). MYO3alpha is highly expreesed in retina and cochlea.

Reconstitución

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Almacenamiento y estabilidad

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Información legal

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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