SRP0728

MYO3ALPHA, Active

PRECISIO^® Kinase, recombinant

Propiedades

• recombinant: expressed in baculovirus infected Sf9 cells
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 3.0-5.0 nmol/min·mg (radiometric)
• mol wt: ~76 kDa
• concentration: 0.1 μg/μL
• accession no.: NM_017433
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MYO3A(53904)

Descripción

Application

Useful for kinase assay and Western blot.

Biochem/physiol Actions

Recombinant human MYO3alpha (1-434) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3alpha belongs to the myosin superfamily which are members of the large myosin superfamily and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as MYO3alpha, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors ⁽¹⁾. MYO3alpha plays an important role in hearing in humans and mutations in the MYO3alpha have been shown to cause nonsyndromic progressive hearing loss ⁽²⁾. MYO3alpha is highly expreesed in retina and cochlea.

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• WGK Germany: WGK 1
• Flash Point F: Not applicable
• Flash Point C: Not applicable