Useful for kinase assay.
Recombinant human PIP5K3 (1400-end) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP5K3 (also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) encodes an enzyme that phosphorylates the D-5 position in PtdIns and phospha-tidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns (3,5)biphosphate. PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol but also have protein kinase activity (1). PIP5K3 regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. The inhibition of PIP5K3 renders the late endosome /lysosome compartment refractory to fusion with autop-hagosomes and multivesicular bodies (2).
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
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