SRP2027

Sigma-Aldrich

E2F-1 (RBAP-1) human

recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

Sinónimos:
E2F-1, RBBP3, RBAP1, RBP3
NACRES:
NA.26
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

human

recombinant

expressed in insect cells

assay

≥70% (SDS-PAGE)

form

frozen liquid

mol wt

~48.6 kDa

packaging

pkg of 5 μg

concentration

400 μg/mL

color

colorless to clear

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... E2F1(189971)

Biochem/physiol Actions

E2F was originally identified as a transcription factor mediating the transcription of adenovirus E2 gene. It is a heterodimer composed of two structurally related subunits, termed E2F and DP. E2F is encoded by at least five genes, E2F-1 through E2F-5, and DP is encoded by at least two genes, DP-1 and DP-2. All these genes (E2F-1 through E2F-5, DP-1 and DP-2) have been considered as members of the E2F gene family because they have a number of common structural and functional characteristics. Among those members of the E2F family, E2F-1 was the first E2F gene cloned and characterized. The E2F-1 protein has been shown to interact with RB both in vitro and in vivo. Although the E2F proteins can function as transcription factors by themselves, they should be considered as RB-mediators as well because almost every functional response of RB protein(s) requires the presence of E2F protein(s).

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
S Bagchi et al.
Cell, 62(4), 659-669 (1990-08-24)
Adenovirus infection activates the E2F transcription factor, in part through the formation of a heteromeric protein complex involving a 19 kd E4 gene product that then allows cooperative and stable promoter binding. We now find that cellular factors are complexed...
W G Kaelin et al.
Cell, 70(2), 351-364 (1992-08-03)
An expression vector was modified to permit the rapid synthesis of purified, 32P-labeled, glutathione S-transferase (GST)-retinoblastoma (RB) fusion proteins. The products were used to screen lambda gt11 expression libraries, from which we cloned a cDNA encoding a polypeptide (RBAP-1) capable...
K Helin et al.
Cell, 70(2), 337-350 (1992-07-24)
The retinoblastoma protein (pRB) plays an important role in the control of cell proliferation, apparently by binding to and regulating cellular transcription factors such as E2F. Here we describe the characterization of a cDNA clone that encodes a protein with...

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