SRP2029

Sigma-Aldrich

Sp1 GC-box binding protein human

≥70% (SDS-PAGE)

NACRES:
NA.26
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

human

assay

≥70% (SDS-PAGE)

form

frozen liquid

mol wt

~80.7 kDa

packaging

pkg of 2 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

100 μg/mL

application(s)

electrophoretic mobility shift assay: suitable

color

clear colorless

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SP1(6667)

General description

Specific protein 1 (Sp1) is a transcription regulator that has a transactivation domain at the N-terminus and a DNA-binding domain at the C-terminus, that contain three zinc fingers (ZF). This transcription factor selectively binds to the hexanucleotide sequence, GGGCGG (GC-box). It belongs to transcription factor family and is located on human chromosome 12q13.

Biochem/physiol Actions

Sp1 was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Subsequently it was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including SV40 early, HIV-1, PDGF-B etc. Sp1 was the first transcription factor to be cloned. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element. Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a well-characterized motif found in several other transcription factors. In addition to transcription, Sp1 function has been linked to cell growth, cancer, Huntington disease and other disorders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation.
Specific protein 1 (Sp1) controls the up- and down-regulation of hCtr1 (human high-affinity copper transporter 1). It controls the expression of several genes that participates in differentiation, cell cycle and oncogenesis.It has the ability to bind GC/GT-rich promoter elements with the help of C(2)H(2)-type zinc fingers at the C-terminal domains.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
New insights into the multiple functions of Spl, a ubiquitous transcription factor.
Parnaik V K
Current Science, 76(2), 166-172 (1999)
An insight into the phylogenetic history of HOX linked gene families in vertebrates
Abbasi AA and Grzeschik KH
BMC Evolutionary Biology (2007)
Sp1 and Sp3 function as key regulators of leukotriene C(4) synthase gene expression in the monocyte-like cell line, THP-1.
Serio KJ et al
American Journal of Respiratory Cell and Molecular Biology, 23(2), 234-240 (2000)
Effects of Cu(II) and cisplatin on the stability of Specific protein 1 (Sp1)-DNA binding: Insights into the regulation of copper homeostasis and platinum drug transport
Yan D, et al.
Journal of Inorganic Biochemistry, 161, 37-39 (2016)
New Frontiers in the Study of Gene Functions (2013)

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