Merck
  • SRP2095
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SRP2095

Sigma-Aldrich

MDM2, HIS tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE), buffered aqueous solution

Synonym(s):
HDMX, MGC5370, MGC71221, hdm2
NACRES:
NA.26

origen biológico

human

recombinante

expressed in E. coli

etiqueta

8-His tagged (C-terminus. )

ensayo

≥80% (SDS-PAGE)

formulario

buffered aqueous solution
frozen liquid

mol peso

~57.8 kDa

envase

pkg of 10 μg

concentración

350 μg/mL

color

clear colorless

Nº de acceso NCBI

Nº de acceso UniProt

enviado en

dry ice

temp. de almacenamiento

−70°C

Gene Information

human ... MDM2(4193)

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SRP0467SRP5129SRP0465
biological source

human

biological source

human

biological source

-

biological source

human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥80% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

form

buffered aqueous solution, frozen liquid

form

aqueous solution

form

buffered aqueous glycerol solution

form

aqueous solution

mol wt

~57.8 kDa

mol wt

15.5 kDa

mol wt

~22 kDa

mol wt

15.8 kDa

packaging

pkg of 10 μg

packaging

pkg of 100 μg

packaging

-

packaging

pkg of 100 μg

Acciones bioquímicas o fisiológicas

Originally discovered as one of three genes amplified on double minute chromosomes in a tumorigenic derivative of NIH 3T3 cells, MDM2 was later shown to possess oncogenic potential when overexpressed. High-level expression of MDM2 has also been shown to confer tumorigenic potential upon nontransformed rodent fibroblasts in athymic nude mice. MDM2 can immortalize rat embryo fibroblasts and can cooperate with activated RAS to transform these cells. Elevated levels of MDM2 protein have been found in a variety of human tumors, most notably in soft tissue sarcomas where up to 30% of primary tumors contain multiple copies of the MDM2 gene. One mechanism by which MDM2 overexpression promotes tumor development is through its ability to bind to the p53 tumor suppressor, thereby blocking the transactivation, cell cycle arrest, and apoptotic functions of p53. MDM2 can inhibit p53 activity in a number of ways including preventing p53 from recruiting TAFs, promoting nuclear export, inhibiting p53 acetylation, and perhaps most importantly by virtue of its function as an E3 ubiquitin ligase with specificity for, among others, p53. In addition to regulating p53 levels by targeting p53 for proteasomal degradation MDM2 also transfers ubiquitin to itself, MDMX, the ß2 adrenergic receptor, glucocorticoid receptor, TIP60, and PCAF.

Forma física

Clear and colorless frozen liquid solution

Nota de preparación

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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