SRP3109

MCP-1/MCAF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(s): CCL2, MCAF, Monocyte Chemotactic Protein-1
• NACRES: NA.32

Properties

• origen biológico: human
• recombinante: expressed in E. coli
• ensayo: ≥98% (HPLC); ≥98% (SDS-PAGE)
• formulario: lyophilized
• mol peso: 8.6 kDa
• envase: pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurezas: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• Nº de acceso UniProt: P13500
• enviado en: wet ice
• temp. de almacenamiento: −20°C
• Gene Information: human ... CCL2(6347)

Description

Descripción general

Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF), also called the C-C motif chemokine ligand 2 (CCL2),[1] is an inflammatory cytokine.[2] The CCL2 gene is mapped to human chromosome 17q12.[1] CCL2 is present in epithelial, endothelial, mesangial and microglial cells.[3] Structurally, it dimerizes and has four regions of the ß-sheet.[3] Recombinant human MCP-1 is a 8.6 kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues.[3] The MCP family cross-reacts across species.

Aplicación

Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) has been used:

• as a chemokine supplement in the alpha minimal essential medium for culturing neurons to test its effect on JmjC histone demethylase (Jmjd2a) mRNA[2]
• as a component of Dulbecco′s Modified Eagle (DMEM) medium, as a positive chemoattractant control in HeLa cells for migration assay[4]
• as a chemoattractant to induced macrophage chemotaxis[5]

Acciones bioquímicas o fisiológicas

Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) acts as a human immunodeficiency virus 1 (HIV-1) suppressor.[3] The MCP-1 acts as a chemoattractant for T cells and natural killer (NK) cells.[1] It also plays a key role in the migration and infiltration of macrophages.[3] CCL2 shows under expression in high-grade ovarian serous carcinomas (HGOSCs) and is expressed less in epithelial ovarian cancer (EOC).[1]

Monocyte chemoattractant protein-1/monocyte chemotactic and activating factor (MCP-1/MCAF) is a member of CC chemokine family. It triggers the activation of monocytes by triggering degranulation as well as respiratory burst activity in monocytes. It facilitates monocytes in inhibiting or enhancing the growth of specific brain tumor cell lines. It is found to be overexpressed in cases of atherosclerosis, idiopathic pulmonary fibrosis, rheumatoid arthritis, and delayed hypersensitivity reactions. It is also implicated in Kawasaki disease.[6]

Secuencia

QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

Forma física

Lyophilized with no additives.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 3
• Punto de inflamabilidad F: Not applicable
• Punto de inflamabilidad C: Not applicable