Merck
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SRP3143

Sigma-Aldrich

Pleiotrophin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
PTN, Heparin-binding growth factor-8(HBGF-8), Osteoblast-Specific Factor-1 (OSF-1), Heparin Affin Regulatory Protein (HARP)
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

ensayo

≥98% (HPLC)
≥98% (SDS-PAGE)

formulario

lyophilized

mol peso

15.4 kDa

envase

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurezas

<0.1 EU/μg endotoxin, tested

color

white

Nº de acceso UniProt

enviado en

wet ice

temp. de almacenamiento

−20°C

Gene Information

human ... PTN(5764)

Descripción general

PTN (pleiotrophin) is a 136-residue, platelet-derived growth factor (PDGF)-inducible, heparin-binding cytokine. It shares ~50% similarity with midkine, a retinoic acid-inducible factor. It is expressed in a tissue and stage dependent manner during development, but in adults, this protein is expressed constitutively, though in a restricted tissue pattern. PTN is a low molecular weight protein composed of two domains linked by disulfide bridges, with three antiparallel β-sheets in each domain. This gene is localized to human chromosome 7q33-q34. Recombinant human Pleiotrophin is a 15.4 kDa protein containing 136 amino acid residues and five intra-molecular disulfide bonds.

Acciones bioquímicas o fisiológicas

PTN (pleiotrophin) is a ligand for receptor protein tyrosine phosphatase (RPTP) β/ζ, and binds with it and inhibits its catalytic capabilities in a “ligand-dependent receptor inactivation”-mechanism, and promotes tyrosine phosphorylation of β-catenin. It acts as a mitogen for fibroblasts, endothelial cells, epithelial cells, and trophoblasts. It promotes neurite outgrowth and glial process outgrowth from neurons and glial progenitor cells, respectively. This protein functions as a protooncogene, and is up-regulated in multiple types of tumors, where it promotes tumor angiogenesis and progression. This protein functions as a crucial mitogen during nerve, bone, vascular, and tumor growth, and has been found to be up-regulated in macrodactyly, identifying it as a candidate gene for macrodactyly.

Secuencia

GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD

Forma física

Lyophilized from 10 mM sodium phosphate, pH 7.5.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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