Merck
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SRP3151

Sigma-Aldrich

SCF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
Steel Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Stem Cell Factor
NACRES:
NA.32

origen biológico

human

recombinante

expressed in E. coli

ensayo

≥98% (HPLC)
≥98% (SDS-PAGE)

formulario

lyophilized

potencia

≤2.0 ng/mL ED50

mol peso

18.4 kDa

envase

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurezas

<0.1 EU/μg endotoxin, tested

color

white

Nº de acceso UniProt

enviado en

wet ice

temp. de almacenamiento

−20°C

Gene Information

human ... KITLG(4254)

Descripción general

SCF (stem cell factor) is a hematopoietic cytokine that exists in both soluble and transmembrane forms, which arise due to alternative splicing. Both these forms are functionally active. This gene is localized to human chromosome 12q22-12q24. Endothelial cells and fibroblasts constitutively produce both transmembrane and soluble forms of SCF. It is also produced by keratinocytes in normal skin, and epithelial cells in the gut, and thymus. Recombinant human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Acciones bioquímicas o fisiológicas

SCF (stem cell factor) exerts its activity by signaling through the c-Kit receptor. They participate in hematopoiesis, and development of mast cells and melanocytes. Mutations in either of these proteins result in various phenotypic disorders, such as varying degrees of macrocytic anemia, suppressed numbers of tissue mast cells, reduced fertility, and decreased pigmentation. Linkage analysis along with whole-exome sequencing, c.286_303delinsT (p.Ser96Ter), shows a heterozygous truncating mutation in this gene linked with congenital and stable non-syndromic unilateral and asymmetric hearing loss. rs4590952 polymorphism in this gene is linked with disruption of p53 function through increase in p53-dependent SCF expression, which results in increased susceptibility to testicular cancer.

Secuencia

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Forma física

Lyophilized from 10 mM Acetic Acid.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

pictogramas

Exclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Skin Irrit. 2

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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