Merck
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SRP3196

Sigma-Aldrich

EGF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
Epidermal Growth Factor, URG, Urogastrone
NACRES:
NA.32

origen biológico

mouse

recombinante

expressed in E. coli

ensayo

≥98% (HPLC)
≥98% (SDS-PAGE)

formulario

lyophilized

potencia

0.1 ng/mL

mol peso

6.0 kDa

envase

pkg of 500 μg

technique(s)

cell culture | mammalian: suitable

impurezas

<0.1 EU/μg endotoxin, tested

color

off-white to yellow

Nº de acceso UniProt

enviado en

wet ice

temp. de almacenamiento

−20°C

Gene Information

mouse ... EGF(13645)

Descripción general

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.

Aplicación

EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells.

Acciones bioquímicas o fisiológicas

EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis.

Secuencia

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Forma física

Lyophilized from 10 mM Sodium Phosphate, pH 8.2.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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