SRP3214

Sigma-Aldrich

I-TAC (CXCL11) from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinónimos:
CXCL11, B-R1
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

mouse

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

10.0-100.0 ng/mL

mol wt

9.0 kDa

packaging

pkg of 20 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... CXCL11(56066)

General description

CXCL11 (C-X-C motif chemokine 11), also referred to as I-TAC (interferon-inducible T-cell α chemoattractant), is from to the CXC chemokine family and is an antagonist for CC chemokine receptor 3 (CCR3) and CCR5. CXCL11 was first identified in astrocytes via cDNA sequencing. It can be induced by IFN (interferon)-γ in different cells. CXCL11 interacts with and signals via CXCR3 (C-X-C chemokine receptor type 3).
Recombinant murine I-TAC is a 9.0kDa protein containing 79 amino acid residues including the four highly conserved cysteine residues present in CXC chemokines.

Biochem/physiol Actions

CXCL11 (C-X-C motif chemokine 11) is involved in the wound repair in skin cells. CXCL11′s interaction with CXCR3 (C-X-C chemokine receptor type 3) polarizes naive T cells into IL (interleukin)-10hi Tregs (regulatory T cells, Tr1) and repolarizes CXCR3+CD4+ EAE (experimental autoimmune encephalomyelitis)-associated effector T cells into IL-10hi Tregs. CXCL11 also enhances Th2 (T helper 2) response in model leishmaniasis system, and thereby regulates the outcome of the immunological pathway. In mice model, CXCL11 is upregulated in RSV (respiratory syncytial virus) and influenza A infections.

Sequence

FLMFKQGRCL CIGPGMKAVK MAEIEKASVI YPSNGCDKVE VIVTMKAHKR QRCLDPRSKQ ARLIMQAIEK KNFLRRQNM

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
CXCL11-dependent induction of FOXP3-negative regulatory T cells suppresses autoimmune encephalomyelitis.
Zohar Y, et al.
The Journal of Clinical Investigation, 124, 2009-2009 (2014)
Characterization of the chemokine CXCL11-heparin interaction suggests two different affinities for glycosaminoglycans.
Severin IC, et al.
The Journal of Biological Chemistry, 285, 17713-17713 (2010)
Regulation of C-X-C chemokine gene expression by keratin 17 and hnRNP K in skin tumor keratinocytes.
Chung BM
The Journal of Cell Biology, 208, 613-613 (2015)
Epidermal expression of I-TAC (Cxcl11) instructs adaptive Th2-type immunity.
Roebrock K, et al.
Faseb Journal, 28, 1724-1724 (2014)
A recombinant vaccinia virus encoding the interferon-inducible T-cell alpha chemoattractant is attenuated in vivo.
Hamilton NH, et al.
Scandinavian Journal of Immunology, 59, 246-246 (2004)

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