SRP3242

Sigma-Aldrich

IL-2 from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinónimos:
T-cell growth factor (TCGF), Aldesleukin
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

rat

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.4 ng/mL ED50

mol wt

15.3 kDa

packaging

pkg of 20 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... IL2(116562)

Categorías relacionadas

General description

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues.

Biochem/physiol Actions

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues.

Sequence

MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAIAQSI ISTM

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 4.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificado de Análisis
K A Smith
Science (New York, N.Y.), 240(4856), 1169-1176 (1988-05-27)
Interleukin-2 (IL-2), the first of a series of lymphocytotrophic hormones to be recognized and completely characterized, is pivotal for the generation and regulation of the immune response. A T lymphocyte product, IL-2 also stimulates T cells to undergo cell cycle...
Nabil Ahmed et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 33(15), 1688-1696 (2015-03-25)
The outcome for patients with metastatic or recurrent sarcoma remains poor. Adoptive therapy with tumor-directed T cells is an attractive therapeutic option but has never been evaluated in sarcoma. We conducted a phase I/II clinical study in which patients with...
Lindsey Chudley et al.
Cancer immunology, immunotherapy : CII, 63(11), 1199-1211 (2014-08-20)
Ex vivo ELISPOT and multimer staining are well-established tests for the assessment of antigen-specific T cells. Many laboratories are now using a period of in vitro stimulation (IVS) to enhance detection. Here, we report the findings of a multi-centre panel...
Luiza M Araujo et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(9), 2412-2422 (2014-06-10)
HLA-B27/human β2 -microglobulin (hβ2 m)-transgenic (B27-transgenic) rats develop an inflammatory disorder resembling spondyloarthritis, with accumulation of proinflammatory Th17 cells. Because Treg cells and Th17 cells have opposing effects in inflammatory disorders, we sought to determine whether biased expansion of Th17...
Fang-Ting Lu et al.
Journal of autoimmunity, 61, 62-72 (2015-06-15)
Thymic CD4(+) FoxP3(+) regulatory T (Treg) cells are critical for the development of immunological tolerance and immune homeostasis and requires contributions of both thymic dendritic and epithelial cells. Although B cells have been reported to be present within the thymus...

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