The FGF4 (fibroblast growth factor 4) gene is mapped to human chromosome 11q13.3. It is expressed during early embryonic development. In adults, it is strongly expressed in the testis. The encoded protein mainly interacts with FGFR1c (FGF receptor 1c) and FGFR2c. It also binds to FGFR3c and FGFR4, but to a lesser extent.
- FGF-4 human has been used to study the effect of FGF4 on the differentiation of PDMCs (placenta-derived multipotent cells) into hepatocyte-like cells.
- It has been used as a growth factor to study the proliferation and differentiation of human bone marrow mesenchymal stem cells (hMSCs) to chondrocytes.
- It has been used to study hepatic differentiation.
Acciones bioquímicas o fisiológicas
FGF4 (fibroblast growth factor 4) was first identified as an oncogene transforming NIH3T3 (mouse embryo fibroblast cell line) cells. It helps in maintaining the self-renewal capability of normal stem cells (for instance, trophoblastic stem cells). It is also required for cellular differentiation, morphogenesis and proliferation. It controls proliferation and differentiation of normal stem cells, progenitor cells and cancer cells.
Lyophilized from 10 mM Sodium Phosphate, pH 7.6 + 100 mM NaCl.
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.