Eotaxin is a member of the CC chemokine family, and belongs to the MCP/eotaxin subfamily. It has a wide range of tissue expression, such as thymus, heart, lungs, lymph nodes, kidney, and intestines. It is also expressed by epithelial cells of lung, pleural mesothelial cells, epithelial cells of bronchial airway, and smooth muscle cells (SMCs).
Acciones bioquímicas o fisiológicas
Eotaxin is a chemoattractant for eosinophils, and is involved inflammatory diseases, such as asthma. It is induced by glucocorticoids and cytokines. Along with interleukin-5 (IL-5) it results in airway hyperresponsiveness (AHR), and hence, lung inflammatory disorders. In humans, it leads to respiratory syncytial virus (RSV) vaccine-enhanced disease, and the eosinophils are also responsible for the elimination of virus. It might also be involved in the pathophysiology of inflammatory bowel diseases which involve eosinophils. It can lead to the production of reactive oxygen species, thus, resulting in damaged tissue. It is overexpressed in multiple diseases such as allergic rhinitis, nasal polyposis, endometriosis and Hodgkin′s disease.
Sterile filtered and then lyophilized with no additives. The protein may appear as a haze or film that is difficult to see at the bottom of the vial.
Reconstitute in water to a concentration of 0.1-1 μg/μL. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.